Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University "Júlio de Mesquita Filho" (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900 Jaboticabal, São Paulo, Brazil.
Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinarian Sciences, São Paulo State University "Júlio de Mesquita Filho" (FCAV/UNESP), Jaboticabal, São Paulo, Brazil.
Anim Reprod Sci. 2021 Nov;234:106870. doi: 10.1016/j.anireprosci.2021.106870. Epub 2021 Oct 14.
The aim was to sonographically evaluate the reproductive tract of bitches during the follicular phase of the estrous cycle using High Density (HD) ultrasonic techniques. Females (n = 8) were evaluated at five different times throughout the follicular phase, as determined by vaginal cytology and blood progesterone concentrations. Ultrasonic exams were performed using the ACUSON S2000/SIEMENS device utilizing a multifrequency HD transducer (5.5-18 MHz). Videos of the ovaries were obtained and recordings were evaluated using a DICOM viewer software for counting and measuring the ovarian structures, which were assigned to groups based on diameter in mm: G1: ≤ 1; G2: from 1.01 to 3.5; G3 from 3.51 to 5.5; G4: from 5.51 to 10. There was a greater uterine thickness with the progression of the follicular phase (P < 0.05). Six distinct regions were identified in the uterine wall. The ovarian dimensions increased (P < 0.05) as stage of the follicular phase advanced. There was fluid detected around the ovaries after ovulation. There was a characteristic fat tissue hyperechogenicity around the ovaries at all timepoints. There was a difference in the number of ovarian structures of each dimension group at each time there were assessments (P < 0.05). There was a difference in diameter of the largest ovarian structure and in average value of wall thickness at all timepoints when there were evaluations (P < 0.05). The HD ultrasonography technique provides for excellent image resolution, allowing for a more precise characterization of the bitch's reproductive structures and changes occurring during the follicular phase of the estrous cycle.
本研究旨在应用高频（HD）超声技术对发情周期中卵泡期母犬的生殖道进行超声评估。通过阴道细胞学和血液孕酮浓度检测，将 8 只母犬在卵泡期的 5 个不同时间点进行评估。使用 ACUSON S2000/SIEMENS 设备，通过多频 HD 换能器（5.5-18 MHz）进行超声检查。获取卵巢的视频，并使用 DICOM 查看器软件对记录进行评估，以对卵巢结构进行计数和测量，这些结构根据直径（mm）分为以下几组：G1：≤1；G2：1.01-3.5；G3：3.51-5.5；G4：5.51-10。随着卵泡期的进展，子宫壁厚度增加（P<0.05）。在子宫壁中识别出 6 个不同的区域。随着卵泡期阶段的进展，卵巢尺寸增加（P<0.05）。排卵后，在卵巢周围检测到液体。在所有时间点，卵巢周围均可见特征性的脂肪组织高回声。在每次评估时，每个尺寸组的卵巢结构数量均存在差异（P<0.05）。在所有评估时间点，最大卵巢结构的直径和壁厚度的平均值均存在差异（P<0.05）。HD 超声技术提供了出色的图像分辨率，能够更精确地描述母犬生殖结构，并能在发情周期的卵泡期期间发生的变化。
