Institute for Scintillation Materials National Academy of Sciences of Ukraine,60 Nauky ave., 61072 Kharkiv, Ukraine.
G. V. Kurdyumov Institute for Metal Physics, National Academy of Sciences of Ukraine, 36 Vernadsky Street, 03142 Kiev, Ukraine.
Nanotechnology. 2021 Nov 8;33(5). doi: 10.1088/1361-6528/ac31e5.
Oxidative stress caused by an overproduction of reactive oxygen species (ROS) is the key factor in developing a variety of pathological conditions. Recently various nanomaterials have attracted growing interest as nanoantioxidants with ROS-regulating ability. Here, for the first time, we report on high antioxidant behavior (enzyme-like activity) of GdYVO:Eunanoparticles (GdYVO NPs) revealed by spectroscopic methods both in cell-free and biological milieu using various ROS sensors. It was revealed that GdYVO NPs (= 2 nm) effectively scavenge hydroxyl radicals·OH,superoxide anionsO2·-,hydrogen peroxideH2O2,peroxyl radicalsROO·,and remarkably reduce the lipopolysaccharide-induced ROS generation in rat leukocytes. The antioxidant activity of GdYVO NPs is ascribed to high amount of Vand Vions in the structure of the NPs and the reversible switchingV3+↔V4+andV4+↔V5+vanadium oxidation states.
活性氧（ROS）的过度产生引起的氧化应激是多种病理状况发展的关键因素。最近，各种纳米材料作为具有 ROS 调节能力的纳米抗氧化剂引起了越来越多的关注。在这里，我们首次通过光谱方法在无细胞和生物环境中使用各种 ROS 传感器报道了 GdYVO：Eunanoparticles（GdYVO NPs）的高抗氧化行为（酶样活性）。结果表明，GdYVO NPs（=2nm）可有效清除羟基自由基·OH、超氧阴离子 O2·-、过氧化氢 H2O2、过氧自由基 ROO·，并显著降低脂多糖诱导的大鼠白细胞中 ROS 的产生。GdYVO NPs 的抗氧化活性归因于 NPs 结构中大量的 Vand Vions 以及可逆的 V3+↔V4+和 V4+↔V5+钒氧化态的转换。
