Operative Unit of Microbiology, University Hospital Policlinico Sant'Orsola-Malpighi, Bologna, Italy.
Probiotical Research s.r.l., Novara, Italy.
Future Microbiol. 2021 Nov;16:1239-1250. doi: 10.2217/fmb-2020-0276. Epub 2021 Oct 22.
is a pathogen of serious concern, often exhibiting multiple antibiotic resistance, frequently associated with hospital outbreaks in intensive care units. A prompt detection and tracking of these isolates is crucial. Reference methods for typing (pulsed-field gel electrophoresis, whole-genome sequencing) are accurate, but expensive and time-consuming, therefore limited to retrospective analysis. In this study, the application of the FTIR-based IR Biotyper (IRBT) to track and monitor in real-time the spread of a multidrug-resistant outbreak was investigated. The index case and the multidrug-resistant isolates collected in the following 3 weeks were investigated. IR Biotyper clustering results were fully confirmed by pulsed-field gel electrophoresis results. IR Biotyper represent a promising tool for real-time hospital hygiene, enabling a prompt and reliable typing.
是一种严重关注的病原体，常表现出多种抗生素耐药性，经常与重症监护病房的医院爆发有关。及时发现和跟踪这些分离株至关重要。用于分型的参考方法（脉冲场凝胶电泳、全基因组测序）准确，但昂贵且耗时，因此仅限于回顾性分析。在这项研究中，应用基于傅里叶变换红外光谱的 IR Biotyper（IRBT）实时跟踪和监测多药耐药性爆发的传播。对索引病例和接下来 3 周内收集的多药耐药分离株进行了研究。IR Biotyper 聚类结果与脉冲场凝胶电泳结果完全吻合。IR Biotyper 代表了一种有前途的实时医院卫生工具，能够实现快速可靠的分型。
