血小板冷冻的改进方法。
Improved procedure for platelet freezing.
作者信息
Dayian G, Harris H L, Vlahides G D, Pert J H
出版信息
Vox Sang. 1986;51(4):292-8. doi: 10.1111/j.1423-0410.1986.tb01971.x.
Platelets frozen in glycerol-glucose frequently aggregated on thawing or reconstitution. This has been eliminated by using a nonplasma diluent (pH 6.5) and a new freezing bag. The results were improved platelet 14C-serotonin uptake and readily demonstrable aggregation to ADP, epinephrine, collagen and thrombin. 14C-serotonin uptake was at maximum when platelets were frozen at thrombocrits of up to 15%. Hemostatic effectiveness was demonstrated in a severely thrombocytopenic patient with acute myelogenous leukemia in relapse, who was supported entirely with two autologous frozen platelet transfusions for the 13 days before bone marrow activity resumed.
甘油 - 葡萄糖冷冻保存的血小板在解冻或复溶时常常发生聚集。通过使用非血浆稀释剂（pH 6.5）和一种新型冷冻袋，这一问题已得到解决。结果显示，血小板对14C - 5 - 羟色胺的摄取有所改善，并且对二磷酸腺苷、肾上腺素、胶原和凝血酶的聚集反应也易于观察到。当血小板在血细胞比容高达15%时进行冷冻，14C - 5 - 羟色胺摄取达到最大值。一名急性髓性白血病复发且严重血小板减少的患者，在骨髓活性恢复前的13天内完全依靠两次自体冷冻血小板输注获得支持，证明了其止血效果。
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