Chinese Medicine Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway.

BACKGROUND

Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine in the pathogenesis of RCC. Meanwhile, this study also revealed the molecular biological mechanism of its antitumor activity.

METHODS

Human RCC 786-O cells were cultured in the RPMI-1640 medium, which contains different concentrations of (1,000, 3,000, and 9,000 g/ml). MTT and flow cytometry assays were used to detect the viability of 786-O cells. Transwell and wound healing assays were used to detect cell metastasis. The protein expression was observed by western blot analysis.

RESULTS

can inhibit cell proliferation and induce apoptosis in RCC 786-O cells. In addition, can weaken the migration and invasion of 786-O cells. More importantly, can block the mTOR pathway by inhibiting the protein expression of p-mTOR. Moreover, the high concentration of has a better inhibitory effect on 786-O cells.

CONCLUSION

The traditional Chinese medicine inhibits the viability and metastasis of 786-O cells by blocking the mTOR pathway.