Division of Molecular Pathology, The Institute of Cancer Research, London, United Kingdom.
Expert Rev Proteomics. 2021 Sep;18(9):781-794. doi: 10.1080/14789450.2021.1984885. Epub 2021 Nov 2.
The matrisome and adhesome comprise proteins that are found within or are associated with the extracellular matrix (ECM) and adhesion complexes, respectively. Interactions between cells and their microenvironment are mediated by key matrisome and adhesome proteins, which direct fundamental processes, including growth and development. Due to their underlying complexity, it has historically been challenging to undertake mass spectrometry (MS)-based profiling of these proteins. New developments in sample preparative workflows, informatics databases, and MS techniques have enabled in-depth proteomic characterization of the matrisome and adhesome, resulting in a comprehensive understanding of the interactomes, and cellular signaling that occur at the cell-ECM interface.
This review summarizes recent advances in proteomic characterization of the matrisome and adhesome. It focuses on the importance of curated databases and discusses key strengths and limitations of different workflows.
MS-based proteomics has shown promise in characterizing the matrisome and topology of adhesome networks in health and disease. Moving forward, it will be important to incorporate integrative analysis to define the bidirectional signaling between the matrisome and adhesome, and adopt new methods for post-translational modification and analyses to better dissect the critical roles that these proteins play in human pathophysiology.
基质体和黏着体分别包含存在于细胞外基质（ECM）内或与之相关的蛋白，以及黏着复合物相关蛋白。细胞与其微环境之间的相互作用由关键的基质体和黏着体蛋白介导，这些蛋白指导包括生长和发育在内的基本过程。由于其潜在的复杂性，基于质谱（MS）的这些蛋白分析在历史上一直具有挑战性。在样品制备工作流程、信息学数据库和 MS 技术方面的新进展，使基质体和黏着体的深入蛋白质组学特征得以实现，从而全面了解细胞-ECM 界面处发生的相互作用和细胞信号转导。
本综述总结了基质体和黏着体蛋白质组学特征分析的最新进展。它侧重于经过策展的数据库的重要性，并讨论了不同工作流程的关键优势和局限性。
基于 MS 的蛋白质组学在健康和疾病中对基质体和黏着体网络的拓扑结构进行特征分析方面显示出了前景。展望未来，重要的是要进行综合分析以定义基质体和黏着体之间的双向信号，以及采用新的翻译后修饰和分析方法来更好地剖析这些蛋白在人类病理生理学中的关键作用。
