Cell Therapy Center, Jinan Central Hospital, Jinan, P.R. China.
Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, P.R. China.
Cell Reprogram. 2021 Dec;23(6):336-348. doi: 10.1089/cell.2021.0043. Epub 2021 Oct 22.
Human mesenchymal stem cells (MSCs) can be isolated from various tissues. However, the cytokine profile in different MSC types remains unclear. In this study, MSCs were extracted from adipose, umbilical cord, and placental tissues. The surface marker expression, multilineage differentiation potential, and cytokine secretion of these cells were compared. The isolated MSCs exhibited similar morphology and surface marker expression. However, they differed with regard to their differentiation potential. Adipose-MSCs (A-MSCs) exhibited a higher potential for adipogenesis and osteogenic differentiation compared with umbilical cord-MSCs (UC-MSCs) and placental-MSCs (P-MSCs). The expression levels of 80 cytokines were detected, and the data demonstrated that the three MSC types abundantly secreted insulin-like growth factor-binding protein (IGFBP)-4, IGFBP-3, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, IGFBP-6, monocyte chemoattractant protein-1, and granulocyte colony-stimulating factor. However, the expression levels of vascular endothelial growth factor, tumor necrosis factor alpha, interleukin (IL)-6 receptor, and IL-13 in A-MSCs were higher compared with those of UC-MSCs and P-MSCs. Moreover, the expression levels of intercellular adhesion molecule-1 and growth differentiation factor 15 were lower in A-MSCs. Kyoto Encyclopedia of Genes and Genomes analysis indicated that the "adipocytokine" and the "PI3K/Akt pathways" were enriched in A-MSCs. Taken together, the results demonstrated that MSCs from different sources exhibited differences in the secretion of specific factors. A-MSCs were associated with the expression of several proangiogenic factors and may be an improved source for angiogenesis and tissue regeneration.
人骨髓间充质干细胞（MSCs）可从各种组织中分离得到。然而，不同类型的 MSCs 的细胞因子谱仍不清楚。在这项研究中，从脂肪、脐带和胎盘组织中提取了 MSCs。比较了这些细胞的表面标志物表达、多能性分化潜能和细胞因子分泌。分离的 MSCs 表现出相似的形态和表面标志物表达。然而，它们在分化潜能上存在差异。脂肪间充质干细胞（A-MSCs）与脐带间充质干细胞（UC-MSCs）和胎盘间充质干细胞（P-MSCs）相比，具有更高的成脂和成骨分化潜能。检测了 80 种细胞因子的表达水平，数据表明这三种 MSC 类型大量分泌胰岛素样生长因子结合蛋白（IGFBP）-4、IGFBP-3、金属蛋白酶组织抑制剂（TIMP）-1、TIMP-2、IGFBP-6、单核细胞趋化蛋白-1 和粒细胞集落刺激因子。然而，A-MSCs 中血管内皮生长因子、肿瘤坏死因子-α、白细胞介素（IL）-6 受体和 IL-13 的表达水平高于 UC-MSCs 和 P-MSCs。此外，A-MSCs 中细胞间黏附分子-1 和生长分化因子 15 的表达水平较低。京都基因与基因组百科全书分析表明，“脂肪细胞因子”和“PI3K/Akt 通路”在 A-MSCs 中富集。综上所述，结果表明不同来源的 MSCs 在特定因子的分泌上存在差异。A-MSCs 与几种促血管生成因子的表达有关，可能是促进血管生成和组织再生的一种改良来源。
