Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Dollygunj, Port Blair 744 103, Andaman and Nicobar Islands, India.
Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Pallikaranai, Chennai 600 100, Tamil Nadu, India.
Mar Drugs. 2021 Oct 14;19(10):571. doi: 10.3390/md19100571.
Thraustochytrids are the most promising microbial source for the commercial production of docosahexaenoic acid (DHA) for its application in the human health, aquaculture, and nutraceutical sectors. The present study isolated 127 thraustochytrid strains from mangrove habitats of the south Andaman Islands, India to study their diversity, polyunsaturated fatty acids (PUFAs), and biotechnological potential. The predominant strains were identified as belonging to two major genera () based on morphological and molecular characteristics. The strain ANVKK-06 produced the maximum biomass of 5.42 g·L, while ANVKK-03 exhibited the maximum total lipid (71.03%). Omega-3 PUFAs such as eicosapentaenoic acid (EPA) accumulated up to 11.03% in ANVKK-04, docosapentaenoic acid (DPA) up to 8.65% in ANVKK-07, and DHA up to 47.19% in ANVKK-06. ANVKK-06 showed the maximum scavenging activity (84.79 ± 2.30%) while ANVKK-03 and ANVKK-10 displayed the highest antibacterial activity against human and fish pathogens, (18.69 ± 1.2 mm) and (18.31 ± 1.0 mm), respectively. All strains were non-toxic as evident by negative blood agar hemolysis, thus, the thraustochytrids are suggested to be a potential source of DHA for application in the health care of human and fish.
厚壳贻贝是最有前途的微生物来源，可用于商业生产二十二碳六烯酸 (DHA)，应用于人类健康、水产养殖和营养保健品领域。本研究从印度南安达曼群岛的红树林栖息地分离了 127 株厚壳贻贝菌株，以研究其多样性、多不饱和脂肪酸 (PUFA) 和生物技术潜力。根据形态学和分子特征，主要菌株被鉴定为属于两个主要属（）。菌株 ANVKK-06 产生的生物量最大，为 5.42 g·L，而 ANVKK-03 表现出最大的总脂质（71.03%）。ω-3 PUFAs，如二十碳五烯酸 (EPA) 在 ANVKK-04 中积累高达 11.03%，二十二碳五烯酸 (DPA) 在 ANVKK-07 中积累高达 8.65%，二十二碳六烯酸 (DHA) 在 ANVKK-06 中积累高达 47.19%。ANVKK-06 表现出最大的清除活性（84.79 ± 2.30%），而 ANVKK-03 和 ANVKK-10 对人类和鱼类病原体表现出最高的抗菌活性，分别为 （18.69 ± 1.2 mm）和 （18.31 ± 1.0 mm）。所有菌株的血琼脂溶血均为阴性，表明无毒性，因此，厚壳贻贝有望成为 DHA 的潜在来源，应用于人类和鱼类的保健。
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