Huang Jianzhong, Aki Tsunehiro, Yokochi Toshihiro, Nakahara Toro, Honda Daiske, Kawamoto Seiji, Shigeta Seiko, Ono Kazuhisa, Suzuki Osamu
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
Mar Biotechnol (NY). 2003 Sep-Oct;5(5):450-7. doi: 10.1007/s10126-002-0110-1.
Seven strains of marine microbes producing a significant amount of docosahexaenoic acid (DHA; C22:6, n-3) were screened from seawater collected in coastal areas of Japan and Fiji. They accumulate their respective intermediate fatty acids in addition to DHA. There are 5 kinds of polyunsaturated fatty acid (PUFA) profiles which can be described as (1) DHA/docosapentaenoic acid (DPA; C22:5, n-6), (2) DHA/DPA/eicosapentaenoic acid (EPA; C20:5, n-3), (3) DHA/EPA, (4) DHA/DPA/EPA/arachidonic acid (AA; C20:4, n-6), and (5) DHA/DPA/EPA/AA/docosatetraenoic acid (C22:4, n-6). These isolates are proved to be new thraustochytrids by their specific insertion sequences in the 18S rRNA genes. The phylogenetic tree constructed by molecular analysis of 18S rRNA genes from the isolates and typical thraustochytrids shows that strains with the same PUFA profile form each monophyletic cluster. These results suggest that the C20-22 PUFA profile may be applicable as an effective characteristic for grouping thraustochytrids.
从日本和斐济沿海地区采集的海水中筛选出了7株能大量产生二十二碳六烯酸（DHA；C22:6，n-3）的海洋微生物。除DHA外，它们还积累各自的中间脂肪酸。存在5种多不饱和脂肪酸（PUFA）谱型，可描述为：（1）DHA/二十二碳五烯酸（DPA；C22:5，n-6），（2）DHA/DPA/二十碳五烯酸（EPA；C20:5，n-3），（3）DHA/EPA，（4）DHA/DPA/EPA/花生四烯酸（AA；C20:4，n-6），以及（5）DHA/DPA/EPA/AA/二十二碳四烯酸（C22:4，n-6）。通过对这些分离株18S rRNA基因中特定插入序列的分析，证实它们是新的破囊壶菌。对分离株和典型破囊壶菌的18S rRNA基因进行分子分析构建的系统发育树表明，具有相同PUFA谱型的菌株形成各自的单系簇。这些结果表明，C20 - 22 PUFA谱型可能作为破囊壶菌分类的有效特征。
