Marine Chemical Resource Development, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
Laboratory of Marine Chemical Resource Development, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
Mar Drugs. 2021 Oct 19;19(10):584. doi: 10.3390/md19100584.
In a previous study, we found that the collagen peptides prepared from the by-products of Bester sturgeon had an inhibitory effect on elevated blood glucose levels in a glucose tolerance test with ICR mice. In the present study, we examine the mechanism of the effect of sturgeon collagen peptides (SCPs) in detail. When glucose was orally administered to mice along with the SCPs, it was found that the glucose remained in the stomach for a longer time. In the above tests, the amount of glucose excreted in the feces of mice also increased. On the contrary, it was revealed that the SCPs have a dipeptidyl-peptidase-IV (DPP-IV) inhibitory ability in an in vitro test. In subsequent oral and intravenous glucose administration tests, glucagon-like peptide-1 (GLP-1) and insulin levels in the blood of mice were maintained at high levels. These results suggested the following three mechanisms: SCPs slow the rate of transportation of glucose from the stomach into the small intestine, resulting in delayed glucose absorption; SCPs suppress the absorption of glucose in the small intestine and excrete it from the body; SCPs inhibit DPP-IV in the blood and maintain a high GLP-1 level in blood, which in turn stimulates insulin secretion.
在之前的研究中，我们发现从白鲟副产物中制备的胶原蛋白肽对 ICR 小鼠葡萄糖耐量试验中升高的血糖水平具有抑制作用。在本研究中，我们详细研究了白鲟胶原蛋白肽（SCPs）的作用机制。当 SCPs 与葡萄糖一起口服给予小鼠时，发现葡萄糖在胃中停留的时间更长。在上述试验中，还发现 SCPs 在体外试验中具有二肽基肽酶-4（DPP-IV）抑制能力。在随后的口服和静脉葡萄糖给药试验中，小鼠血液中的胰高血糖素样肽-1（GLP-1）和胰岛素水平保持在较高水平。这些结果表明存在以下三种机制：SCPs 减缓了葡萄糖从胃到小肠的输送速度，导致葡萄糖吸收延迟；SCPs 抑制了小肠对葡萄糖的吸收并将其从体内排出；SCPs 抑制了血液中的 DPP-IV，维持了血液中高 GLP-1 水平，进而刺激胰岛素分泌。
