Department of Pharmaceutical Sciences, University of Milan, 20133 Milan, Italy.
Nutrients. 2020 Mar 18;12(3):794. doi: 10.3390/nu12030794.
(spirulina) is a cyanobacterium, which contains mainly two phycobiliproteins (PBP), i.e., C-phycocyanin (C-PC) and allophycocyanin (APC). In this study, PBP were hydrolyzed using trypsin, and the composition of the hydrolysate was characterized by HPLC-ESI-MS/MS. Furthermore, the potential anti-diabetic activity was assessed by using either biochemical or cellular techniques. Findings suggest that PBP peptides inhibit DPP-IV activity in vitro with a dose-response trend and an IC value falling in the range between 0.5 and 1.0 mg/mL. A lower inhibition of the DPP-IV activity expressed by Caco-2 cells was observed, which was explained by a secondary metabolic degradation exerted by the same cells.
（螺旋藻）是一种蓝藻，主要含有两种藻蓝蛋白（PBP），即藻蓝蛋白（C-PC）和别藻蓝蛋白（APC）。本研究采用胰蛋白酶水解 PBP，并用 HPLC-ESI-MS/MS 对水解产物的组成进行了表征。此外，还采用生化或细胞技术评估了其潜在的抗糖尿病活性。结果表明，PBP 肽对 DPP-IV 活性具有体外抑制作用，呈剂量反应趋势，IC 值在 0.5 至 1.0mg/mL 范围内。Caco-2 细胞的 DPP-IV 活性抑制作用较低，这可以用相同细胞产生的二次代谢降解来解释。
