Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang, China.
College of Tobacco Science, Guizhou University, Guiyang, China.
Genome Biol Evol. 2021 Nov 5;13(11). doi: 10.1093/gbe/evab236.
The green rice leafhopper, Nephotettix cincticeps (Uhler), is an important rice pest and a vector of the rice dwarf virus in Asia. Here, we produced a high-quality chromosome-level genome assembly of 753.23 Mb using PacBio (∼110×) and Hi-C data (∼94×). It contained 163 scaffolds and 950 contigs, whose scaffold/contig N50 lengths reached 85.36/2.57 Mb. And 731.19 Mb (97.07%) of the assembly was anchored into eight pseudochromosomes. Genome completeness was attained to 97.0% according to the insect reference Benchmarking Universal Single-Copy Orthologs (BUSCO) gene set (n = 1,367). We masked 347.10 Mb (46.08%) of the genome as repetitive elements. Nine hundred sixty-two noncoding RNAs were identified and 14,337 protein-coding genes were predicted. We also assigned GO term and KEGG pathway annotations for 10,049 and 9,251 genes, respectively. Significantly expanded gene families were primarily involved in immunity, cuticle, digestion, detoxification, and embryonic development. This study provided a crucial genomic resource for better understanding on the biology and evolution in family Cicadellidae.
绿叶蝉，Nephotettix cincticeps（Uhler），是亚洲重要的水稻害虫和水稻矮缩病毒的载体。在这里，我们使用 PacBio（约 110×）和 Hi-C 数据（约 94×）生成了一个高质量的 753.23Mb 染色体水平基因组组装。它包含 163 个支架和 950 个 contigs，其支架/contig N50 长度分别达到 85.36/2.57Mb。并且组装的 731.19Mb（97.07%）被锚定到八个假染色体上。根据昆虫参考基准通用单拷贝同源物（BUSCO）基因集（n=1367），基因组完整性达到 97.0%。我们屏蔽了 347.10Mb（46.08%）的基因组作为重复元件。鉴定了 962 个非编码 RNA 和 14337 个蛋白质编码基因。我们还分别为 10049 个和 9251 个基因分配了 GO 术语和 KEGG 途径注释。显著扩展的基因家族主要参与免疫、角质层、消化、解毒和胚胎发育。这项研究为更好地了解蝉科家族的生物学和进化提供了重要的基因组资源。
