Shimura Soichiro, Matsumoto Kazumasa, Shimizu Yuriko, Mochizuki Kohei, Shiono Yutaka, Hirano Shuhei, Koguchi Dai, Ikeda Masaomi, Sato Yuichi, Iwamura Masatsugu
Department of Urology, Kitasato University School of Medicine, Sagamihara Campus, 1-15-1 Kitasato, Sagamihara 252-0374, Kanagawa, Japan.
International University of Health and Welfare Atami Hospital, 13-1 Higashikaigan Town, Atami 413-0012, Shizuoka, Japan.
Cancers (Basel). 2021 Oct 14;13(20):5150. doi: 10.3390/cancers13205150.
Tumor markers that can be detected at an early stage are needed. Here, we evaluated the epiplakin expression levels in sera from patients with bladder cancer (BC). Using a micro-dot blot array, we evaluated epiplakin expression levels in 60 patients with BC, 20 patients with stone disease, and 28 healthy volunteers. The area under the curve (AUC) and best cut-off point were calculated using receiver-operating characteristic (ROC) analysis. Serum epiplakin levels were significantly higher in patients with BC than in those with stone disease ( = 0.0013) and in healthy volunteers ( < 0.0001). The AUC-ROC level for BC was 0.78 (95% confidence interval (CI) = 0.69-0.87). Using a cut-off point of 873, epiplakin expression levels exhibited 68.3% sensitivity and 79.2% specificity for BC. However, the serum epiplakin levels did not significantly differ by sex, age, pathological stage and grade, or urine cytology. We performed immunohistochemical staining using the same antibody on another cohort of 127 patients who underwent radical cystectomy. Univariate and multivariate analysis results showed no significant differences between epiplakin expression, clinicopathological findings, and patient prognoses. Our results showed that serum epiplakin might be a potential serodiagnostic biomarker in patients with BC.
需要能够在早期检测到的肿瘤标志物。在此，我们评估了膀胱癌（BC）患者血清中的表皮 plak 蛋白表达水平。使用微点印迹阵列，我们评估了60例BC患者、20例结石病患者和28名健康志愿者的表皮 plak 蛋白表达水平。使用受试者操作特征（ROC）分析计算曲线下面积（AUC）和最佳截断点。BC患者的血清表皮 plak 蛋白水平显著高于结石病患者（=0.0013）和健康志愿者（<0.0001）。BC的AUC-ROC水平为0.78（95%置信区间（CI）=0.69-0.87）。使用873的截断点，表皮 plak 蛋白表达水平对BC的敏感性为68.3%，特异性为79.2%。然而，血清表皮 plak 蛋白水平在性别、年龄、病理分期和分级或尿液细胞学方面没有显著差异。我们对另一组127例行根治性膀胱切除术的患者使用相同抗体进行了免疫组化染色。单因素和多因素分析结果显示，表皮 plak 蛋白表达、临床病理结果和患者预后之间无显著差异。我们的结果表明，血清表皮 plak 蛋白可能是BC患者潜在的血清诊断生物标志物。
