Munguti Florence M, Kilalo Dora C, Nyaboga Evans N, Wosula Everlyne N, Macharia Isaac, Mwango'mbe Agnes W
Department of Plant Science and Crop Protection, University Nairobi, Kangemi, Nairobi P.O. Box 29053-00625, Kenya.
Kenya Plant Health Inspectorate Service, GPO, Nairobi P.O. Box 49592-00100, Kenya.
Insects. 2021 Sep 27;12(10):875. doi: 10.3390/insects12100875.
The whitefly, (Gennadium, Hemiptera) has been reported to transmit viruses that cause cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) in many parts of sub-Saharan Africa (SSA). Currently, there is limited information on the distribution, species and haplotype composition of the whitefly populations colonizing cassava in Kenya. A study was conducted in the major cassava growing regions of Kenya to address this gap. Analyses of mitochondrial DNA cytochrome oxidase 1 (mtCO1) sequences revealed the presence of four distinct whitefly species: , , and in Kenya. The haplotypes were further resolved into SSA1, SSA2 and Indian Ocean (IO) putative species. The SSA1 population had three haplogroups of SSA1-SG1, SSA-SG2 and SSA1-SG3. Application of KASP genotyping grouped the into two haplogroups namely sub-Saharan Africa East and Southern Africa (SSA-ESA) and sub-Saharan Africa East and Central Africa (SSA-ECA). The study presents the first report of (Bondar's nesting whitefly) on cassava in Kenya. was widely distributed in all the major cassava growing regions in Kenya. The increased detection of different whitefly species on cassava and genetically diverse mitotypes indicates a significant influence on the dynamics of cassava virus epidemics in the field. The study highlights the need for continuous monitoring of invasive whitefly species population on cassava for timely application of management practices to reduce the impact of cassava viral diseases and prevent potential yield losses.
粉虱（烟粉虱属，半翅目）在撒哈拉以南非洲（SSA）的许多地区被报道可传播导致木薯花叶病（CMD）和木薯褐色条纹病（CBSD）的病毒。目前，关于肯尼亚木薯上粉虱种群的分布、种类和单倍型组成的信息有限。在肯尼亚主要的木薯种植区开展了一项研究以填补这一空白。对线粒体DNA细胞色素氧化酶1（mtCO1）序列的分析揭示了肯尼亚存在四种不同的粉虱种类：烟粉虱、温室粉虱、甘薯粉虱和邦氏粉虱。邦氏粉虱的单倍型进一步被解析为SSA1、SSA2和印度洋（IO）假定种类。SSA1种群有三个单倍群，即SSA1-SG1、SSA-SG2和SSA1-SG3。KASP基因分型的应用将邦氏粉虱分为两个单倍群，即撒哈拉以南非洲东部和南部（SSA-ESA）以及撒哈拉以南非洲东部和中部（SSA-ECA）。该研究首次报道了邦氏粉虱（邦达尔嵌套粉虱）在肯尼亚木薯上的情况。邦氏粉虱在肯尼亚所有主要的木薯种植区广泛分布。在木薯上检测到不同粉虱种类以及基因多样的邦氏粉虱线粒体类型的增加，表明其对田间木薯病毒流行动态有重大影响。该研究强调需要持续监测木薯上入侵粉虱种类的种群，以便及时应用管理措施，减少木薯病毒病的影响并防止潜在的产量损失。
