Fang Shou-Min, Zhang Qian, Zhang Yu-Li, Zhang Gui-Zheng, Zhang Ze, Yu Quan-You
School of Life Sciences, Chongqing University, Chongqing 400044, China.
College of Life Science, China West Normal University, Nanchong 637002, China.
Insects. 2021 Oct 12;12(10):928. doi: 10.3390/insects12100928.
The 70 kDa heat shock proteins play important roles in protecting organisms against environmental stresses, which are divided into stress-inducible forms (HSP70s) and heat shock cognates (HSC70s). In this study, heat shock protein 70 family was identified in the whole genome of the silkworm. Based on the known nomenclature and phylogenetic analysis, four HSP70s and five HSC70s were classified. Relatively, heat shock cognates were more conservative and were constitutively expressed in various tissues of the silkworm larvae. Under thermal (37 °C and 42 °C) and cold (2 °C) stresses, the expressions of , , and were up-regulated, and the highest induction reached 4147.3, 607.1, and 1987.3 times, respectively. Interestingly, , , and also showed slight induced expressions in the fat body and/or midgut under thermal stresses. In addition, the expression of was induced by dichlorvos and phoxim insecticides, while most HSC70 genes were inhibited. The results suggested that stress-inducible forms play more important roles in adaptation to various stresses than HSC70s.
70 kDa热休克蛋白在保护生物体免受环境应激方面发挥着重要作用，它们分为应激诱导型（HSP70s）和热休克同源蛋白（HSC70s）。在本研究中，在家蚕全基因组中鉴定出热休克蛋白70家族。基于已知的命名法和系统发育分析，分类出了4个HSP70s和5个HSC70s。相对而言，热休克同源蛋白更为保守，在家蚕幼虫的各种组织中组成性表达。在热应激（37℃和42℃）和冷应激（2℃）下，、和的表达上调，最高诱导倍数分别达到4147.3、607.1和1987.3倍。有趣的是，在热应激下，、和在家蚕脂肪体和/或中肠中也表现出轻微的诱导表达。此外，敌敌畏和辛硫磷杀虫剂可诱导的表达，而大多数HSC70基因受到抑制。结果表明，应激诱导型在适应各种应激方面比HSC70s发挥着更重要的作用。
