College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Theor Appl Genet. 2024 Jun 5;137(7):149. doi: 10.1007/s00122-024-04656-3.
Analyze the evolutionary pattern of DNAJ protein genes in the Panicoideae, including pearl millet, to identify and characterize the biological function of PgDNAJ genes in pearl millet. Global warming has become a major factor threatening food security and human development. It is urgent to analyze the heat-tolerant mechanism of plants and cultivate crops that are adapted to high temperature conditions. The Panicoideae are the second largest subfamily of the Poaceae, widely distributed in warm temperate and tropical regions. Many of these species have been reported to have strong adaptability to high temperature stress, such as pearl millet, foxtail millet and sorghum. The evolutionary differences in DNAJ protein genes among 12 Panicoideae species and 10 other species were identified and analyzed. Among them, 79% of Panicoideae DNAJ protein genes were associated with retrotransposon insertion. Analysis of the DNAJ protein pan-gene family in six pearl millet accessions revealed that the non-core genes contained significantly more TEs than the core genes. By identifying and analyzing the distribution and types of TEs near the DNAJ protein genes, it was found that the insertion of Copia and Gypsy retrotransposons provided the source of expansion for the DNAJ protein genes in the Panicoideae. Based on the analysis of the evolutionary pattern of DNAJ protein genes in Panicoideae, the PgDNAJ was obtained from pearl millet through identification. PgDNAJ reduces the accumulation of reactive oxygen species caused by high temperature by activating ascorbate peroxidase (APX), thereby improving the heat resistance of plants. In summary, these data provide new ideas for mining potential heat-tolerant genes in Panicoideae, and help to improve the heat tolerance of other crops.
分析 Panicoideae 中 DNAJ 蛋白基因的进化模式，包括珍珠粟，以鉴定和表征珍珠粟中 PgDNAJ 基因的生物学功能。全球变暖已成为威胁粮食安全和人类发展的主要因素。迫切需要分析植物的耐热机制，并培育适应高温条件的作物。Panicoideae 是禾本科的第二大亚科，广泛分布于暖温带和热带地区。许多报道称，这些物种对高温胁迫具有很强的适应性，如珍珠粟、谷子和高粱。鉴定和分析了 12 种 Panicoideae 物种和其他 10 种物种的 DNAJ 蛋白基因的进化差异。其中，79%的 Panicoideae DNAJ 蛋白基因与逆转座子插入有关。对 6 个珍珠粟品系的 DNAJ 蛋白泛基因家族的分析表明，非核心基因比核心基因含有更多的 TEs。通过鉴定和分析 DNAJ 蛋白基因附近的 TEs 分布和类型，发现 Copia 和 Gypsy 逆转座子的插入为 Panicoideae 中的 DNAJ 蛋白基因的扩张提供了来源。基于 Panicoideae DNAJ 蛋白基因进化模式的分析，从珍珠粟中获得了 PgDNAJ。PgDNAJ 通过激活抗坏血酸过氧化物酶（APX）减少高温引起的活性氧积累，从而提高植物的耐热性。总之，这些数据为挖掘 Panicoideae 中潜在的耐热基因提供了新的思路，并有助于提高其他作物的耐热性。
