Chekabab Samuel M, Lawrence John R, Alvarado Alvin C, Predicala Bernardo Z, Korber Darren R
Food and Bioproduct Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada.
Prairie Swine Centre Inc., Box 21057, 2105-8th Street East, Saskatoon, SK S7H 5N9, Canada.
Antibiotics (Basel). 2021 Sep 24;10(10):1152. doi: 10.3390/antibiotics10101152.
In response to new stringent regulations in Canada regarding the use of antibiotics in animal production, many farms have implemented practices to produce animals that are raised without antibiotics (RWA) from birth to slaughter. This study aims to assess the impact of RWA production practices on reducing the actual total on-farm use of antibiotics, the occurrence of pathogens, and the prevalence of antimicrobial resistance (AMR). A 28-month longitudinal surveillance of farms that adopted the RWA program and conventional farms using antibiotics in accordance with the new regulations (non-RWA) was conducted by collecting fecal samples from 6-week-old pigs and composite manure from the barn over six time points and applying whole-genome sequencing (WGS) to assess the prevalence of AMR genes as well as the abundance of pathogens. Analysis of in-barn drug use records confirmed the decreased consumption of antibiotics in RWA barns compared to non-RWA barns. WGS analyses revealed that RWA barns had reduced the frequency of AMR genes in piglet feces and in-barn manure. However, metagenomic analyses showed that RWA barns had a significant increase in the frequency of pathogenic in fecal samples and pathogenic in barn manure samples.
为应对加拿大对动物生产中使用抗生素出台的新严格规定，许多养殖场已实施相关做法，以生产从出生到屠宰都不使用抗生素（RWA）饲养的动物。本研究旨在评估RWA生产方式对减少农场抗生素实际总用量、病原体出现情况以及抗菌药物耐药性（AMR）流行率的影响。通过在六个时间点收集6周龄仔猪的粪便样本和猪舍的混合粪便，并应用全基因组测序（WGS）来评估AMR基因的流行率以及病原体的丰度，对采用RWA计划的养殖场和按照新规定使用抗生素的传统养殖场（非RWA）进行了为期28个月的纵向监测。对猪舍内药物使用记录的分析证实，与非RWA猪舍相比，RWA猪舍的抗生素消耗量有所减少。WGS分析表明，RWA猪舍降低了仔猪粪便和猪舍粪便中AMR基因的频率。然而，宏基因组分析显示，RWA猪舍粪便样本中病原菌的频率和猪舍粪便样本中病原菌的频率显著增加。
