Institute of Pathology, Faculty of Medicine, University of Ljubljana, Korytkova 2, SI-1000 Ljubljana, Slovenia.
Agricultural Institute of Slovenia, Hacquetova Ulica 17, SI-1000 Ljubljana, Slovenia.
Genes (Basel). 2021 Sep 23;12(10):1477. doi: 10.3390/genes12101477.
Ulcerative colitis (UC) and Crohn's disease (CD) are characterized by an imbalance between pro-inflammatory and anti-inflammatory cytokines, interfering with the resolution of inflammation. Due to the crucial role of cytokines, new insights into their profiles in UC and CD would help to improve our understanding of pathogenesis and enable the development of new treatment modalities. We provide an expression profile of cytokines in UC and CD, using bioinformatics approach, and experimental validation of expression of the selected genes. We retrieved data and analyzed the cytokine gene expression profiles of UC and CD. From ten genes with inverse expression, common to CD and UC, , and were selected for gene expression experimental validation. Experimentally, and were down-regulated in both CD and UC but followed the bioinformatics trend. The expression of genes and was statistically significant when comparing UC and CD in colon and the expression of gene showed statistical significance when CD in ileum and colon were compared. Using the bioinformatics approach and experimental validation, we found differences in expression profiles between UC and CD for , and . These three promising candidate genes need to be further explored at different levels, such as DNA methylation and protein expression, to provide more evidence on their potential diagnostic role in CD and UC.
溃疡性结肠炎（UC）和克罗恩病（CD）的特征是促炎细胞因子和抗炎细胞因子之间失衡，干扰炎症的消退。由于细胞因子的关键作用，对 UC 和 CD 中细胞因子谱的新见解将有助于提高我们对发病机制的理解，并能够开发新的治疗方式。我们使用生物信息学方法提供了 UC 和 CD 中细胞因子的表达谱，并对选定基因的表达进行了实验验证。我们检索了数据并分析了 UC 和 CD 的细胞因子基因表达谱。从 CD 和 UC 共有的十个表达相反的基因中，选择了 、 和 进行基因表达实验验证。实验结果表明， 和 在 CD 和 UC 中均下调，但符合生物信息学趋势。在比较 UC 和 CD 时，基因 和 的表达具有统计学意义，而当比较 CD 回肠和结肠时，基因 的表达具有统计学意义。通过生物信息学方法和实验验证，我们发现 UC 和 CD 之间在 、 和 方面的表达谱存在差异。这三个有前途的候选基因需要在不同水平上进一步探索，例如 DNA 甲基化和蛋白质表达，以提供更多关于它们在 CD 和 UC 中潜在诊断作用的证据。
