Institute of Marine and Environmental Sciences, University of Szczecin, Adama Mickiewicza 16 St., 70-383 Szczecin, Poland.
Polish Society for Conservation Genetics LUTREOLA, Maciejkowa 21 St., 71-784 Szczecin, Poland.
Genes (Basel). 2021 Sep 29;12(10):1555. doi: 10.3390/genes12101555.
Although properly designed sampling in population genetic studies is of key importance for planning evidence-informed conservation measures, sampling strategies are rarely discussed. This is the case for the European mink , a critically endangered species. In order to address this problem, a meta-analysis aiming to examine the completeness of mtDNA haplotype sampling in recent studies of inter-population genetic diversity was conducted. The analysis was performed using the sample-size-based rarefaction and extrapolation sampling curve method for three populations-the Northeastern (Russia, Belarus and Estonia), the Western (France and Spain), and the Southeastern (Romania). The extrapolated values of the Shannon-Wiener index were determined, assuming full sample coverage. The gap between the measured and predicted inter-population genetic diversity was estimated, indicating that the identified level of sample coverage was the lowest for the NE population (87%), followed by the SE population (96%) and the W population (99%). A guide for sampling design and accounting for sampling uncertainty in future population genetic studies on European mink is provided. The relatively low sample coverage for the Russian population clearly indicates an urgent need to take conservation measures for European mink in this country.
尽管在群体遗传学研究中进行适当的抽样对于规划基于证据的保护措施至关重要，但抽样策略很少被讨论。这就是极度濒危物种欧洲貂的情况。为了解决这个问题，进行了一项荟萃分析，旨在检查最近关于种群间遗传多样性的研究中 mtDNA 单倍型抽样的完整性。该分析使用基于样本量的稀疏和外推抽样曲线方法对三个种群进行了分析——东北（俄罗斯、白俄罗斯和爱沙尼亚）、西部（法国和西班牙）和东南（罗马尼亚）。根据完整的样本覆盖，确定了香农-威纳指数的外推值。测量的和预测的种群间遗传多样性之间的差距表明，东北种群的样本覆盖率最低（87%），其次是东南种群（96%）和西部种群（99%）。为未来的欧洲貂种群遗传学研究提供了抽样设计和考虑抽样不确定性的指南。俄罗斯种群的样本覆盖率相对较低，这清楚地表明该国急需采取保护措施来保护欧洲貂。
