Wipplinger Martin, Mink Sylvia, Bublitz Maike, Gassner Christoph
Institute of Translational Medicine, Private University in the Principality of Liechtenstein, Triesen, Liechtenstein.
Central Medical Laboratories, Feldkirch, Austria.
Transfus Med Hemother. 2024 Jun 19;51(4):225-236. doi: 10.1159/000538863. eCollection 2024 Aug.
The Lewis (Le) blood group system, unlike most other blood groups, is not defined by antigens produced internally to the erythrocytes and their precursors but rather by glycan antigens adsorbed on to the erythrocyte membrane from the plasma. These oligosaccharides are synthesized by the two fucosyltransferases and mainly in epithelial cells of the digestive tract and transferred to the plasma. At their place of synthesis, some Lewis blood group carbohydrate antigen variants also seem to be involved in various gastrointestinal malignancies. However, relatively little is known about the transcriptional regulation of and .
To address this question, we screened existing literature and additionally used in silico prediction tools to identify novel candidate regulators for and and combine these findings with already known data on their regulation. With this approach, we were able to describe a variety of transcription factors, RNA binding proteins and microRNAs, which increase and transcription and translation upon interaction.
Understanding the regulation of and is crucial to fully understand the blood group system Lewis (ISBT 007 LE) phenotypes, to shed light on the role of the different Lewis antigens in various pathologies, and to identify potential new diagnostic targets for these diseases.
与大多数其他血型系统不同，刘易斯（Le）血型系统并非由红细胞及其前体内部产生的抗原所定义，而是由从血浆吸附到红细胞膜上的聚糖抗原所定义。这些寡糖主要由两种岩藻糖基转移酶在消化道上皮细胞中合成并转移到血浆中。在其合成部位，一些刘易斯血型碳水化合物抗原变体似乎也与各种胃肠道恶性肿瘤有关。然而，关于 和 的转录调控相对知之甚少。
为了解决这个问题，我们筛选了现有文献，并额外使用了计算机预测工具来识别 和 的新型候选调节因子，并将这些发现与它们调控的已知数据相结合。通过这种方法，我们能够描述多种转录因子、RNA结合蛋白和微小RNA，它们在相互作用时会增加 和 的转录及翻译。
了解 和 的调控对于全面理解刘易斯血型系统（国际输血协会007 LE）的表型、阐明不同刘易斯抗原在各种病理中的作用以及识别这些疾病潜在的新诊断靶点至关重要。
