Ding Xiaojie, Li Xinchun, Li Yan, Guo Huiyan, Cao Kexin, An Mengnan, Zhang Chong, Wu Yuanhua, Liu He, Zhou Rujun
College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, Liaoning, PR China.
National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang, 550025, PR China.
Mater Today Bio. 2025 Jul 16;34:102099. doi: 10.1016/j.mtbio.2025.102099. eCollection 2025 Oct.
Kühn is an important fungal pathogen that causes serious crop yield losses worldwide. The application of nanoscale strategies based on RNA interference (RNAi) represents an environmentally friendly and efficient approach for plant disease control. In this study, glycosyl hydrolase family 1 (), which functions as a cell wall degrading enzyme (CWDE), and was screened as a prospective RNAi target gene for managing AG3 TB. Additionally, a novel nanosystem for loading and stabilization of double-stranded RNA (dsRNA) was developed. When the mass ratio of ε-poly-L-lysine (ε-PL) to carboxymethyl chitosan (CMCS) is 1:1, ε-PL can spontaneously conjugate with CMCS to form nanoscale spherical particles by electrostatic interaction, hydrogen bonding and Van der Waals forces. Compared with ε-PL alone, the introduction of CMCS resulted in more regular and uniform nanoparticles. ds spontaneously binds with ε-PL@CMCS, which is referred to as ds@ε-PL@CMCS. ε-PL@CMCS protected dsRNA from RNase A degradation effectively. The combination of ds with ε-PL@CMCS remarkably improved the deposition and adhesion of ds droplets on () leaves. The application of ε-PL@CMCS improved the RNAi efficiency of ds and prolonged its protective duration on crops. ds derived from AG3 TB also exhibited broad-spectrum activity against AG1-IA in rice and maize plants. In this study, a self-assembled multi-component nano-fungicide was designed based on dsRNA and nanocarriers. This work proposes an eco-friendly strategy to manage .
库恩菌是一种重要的真菌病原体，在全球范围内导致严重的作物产量损失。基于RNA干扰（RNAi）的纳米级策略的应用是一种环境友好且高效的植物病害防治方法。在本研究中，糖基水解酶家族1（）作为一种细胞壁降解酶（CWDE）发挥作用，并被筛选为用于防治AG3 TB的潜在RNAi靶基因。此外，还开发了一种用于双链RNA（dsRNA）负载和稳定化的新型纳米系统。当ε-聚-L-赖氨酸（ε-PL）与羧甲基壳聚糖（CMCS）的质量比为1:1时，ε-PL可通过静电相互作用、氢键和范德华力与CMCS自发共轭形成纳米级球形颗粒。与单独的ε-PL相比，CMCS的引入导致纳米颗粒更规则、更均匀。dsRNA与ε-PL@CMCS自发结合，称为ds@ε-PL@CMCS。ε-PL@CMCS有效保护dsRNA免受核糖核酸酶A的降解。dsRNA与ε-PL@CMCS的组合显著提高了dsRNA液滴在（）叶片上的沉积和附着力。ε-PL@CMCS的应用提高了dsRNA的RNAi效率，并延长了其在作物上的保护持续时间。源自AG3 TB的dsRNA在水稻和玉米植株中对AG1-IA也表现出广谱活性。在本研究中，基于dsRNA和纳米载体设计了一种自组装多组分纳米杀菌剂。这项工作提出了一种生态友好型策略来防治。
