A novel self-assembled nanocarrier-mediated dsRNA fungicide for broad-spectrum management of .

Kühn is an important fungal pathogen that causes serious crop yield losses worldwide. The application of nanoscale strategies based on RNA interference (RNAi) represents an environmentally friendly and efficient approach for plant disease control. In this study, glycosyl hydrolase family 1 (), which functions as a cell wall degrading enzyme (CWDE), and was screened as a prospective RNAi target gene for managing AG3 TB. Additionally, a novel nanosystem for loading and stabilization of double-stranded RNA (dsRNA) was developed. When the mass ratio of ε-poly-L-lysine (ε-PL) to carboxymethyl chitosan (CMCS) is 1:1, ε-PL can spontaneously conjugate with CMCS to form nanoscale spherical particles by electrostatic interaction, hydrogen bonding and Van der Waals forces. Compared with ε-PL alone, the introduction of CMCS resulted in more regular and uniform nanoparticles. ds spontaneously binds with ε-PL@CMCS, which is referred to as ds@ε-PL@CMCS. ε-PL@CMCS protected dsRNA from RNase A degradation effectively. The combination of ds with ε-PL@CMCS remarkably improved the deposition and adhesion of ds droplets on () leaves. The application of ε-PL@CMCS improved the RNAi efficiency of ds and prolonged its protective duration on crops. ds derived from AG3 TB also exhibited broad-spectrum activity against AG1-IA in rice and maize plants. In this study, a self-assembled multi-component nano-fungicide was designed based on dsRNA and nanocarriers. This work proposes an eco-friendly strategy to manage .