Niu Lili, Sulek Karolina, Vasilopoulou Catherine G, Santos Alberto, Wewer Albrechtsen Nicolai J, Rasmussen Simon, Meier Florian, Mann Matthias
Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
J Clin Med. 2021 Oct 12;10(20):4673. doi: 10.3390/jcm10204673.
Non-alcoholic steatohepatitis (NASH) is a chronic liver disease affecting up to 6.5% of the general population. There is no simple definition of NASH, and the molecular mechanism underlying disease pathogenesis remains elusive. Studies applying single omics technologies have enabled a better understanding of the molecular profiles associated with steatosis and hepatic inflammation-the commonly accepted histologic features for diagnosing NASH, as well as the discovery of novel candidate biomarkers. Multi-omics analysis holds great potential to uncover new insights into disease mechanism through integrating multiple layers of molecular information. Despite the technical and computational challenges associated with such efforts, a few pioneering studies have successfully applied multi-omics technologies to investigate NASH. Here, we review the most recent technological developments in mass spectrometry (MS)-based proteomics, metabolomics, and lipidomics. We summarize multi-omics studies and emerging omics biomarkers in NASH and highlight the biological insights gained through these integrated analyses.
非酒精性脂肪性肝炎(NASH)是一种慢性肝病,影响着高达6.5%的普通人群。NASH没有简单的定义,疾病发病机制的分子机制仍然难以捉摸。应用单一组学技术的研究有助于更好地理解与脂肪变性和肝脏炎症相关的分子特征——这是诊断NASH普遍接受的组织学特征,同时也有助于发现新的候选生物标志物。多组学分析通过整合多层分子信息,在揭示疾病机制新见解方面具有巨大潜力。尽管此类研究存在技术和计算方面的挑战,但一些开创性研究已成功应用多组学技术来研究NASH。在此,我们综述基于质谱(MS)的蛋白质组学、代谢组学和脂质组学的最新技术进展。我们总结了NASH中的多组学研究和新兴的组学生物标志物,并强调通过这些综合分析获得的生物学见解。