Xu Weifang, Wang Fei, Wang Ruolin, Sui Yuan, Zhou Zeyang, Xie Jie, Xiang Zhonghuai
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Sericultural Biology and Genetic Breeding in Ministry of Agriculture, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China.
Anhui Province Key Laboratory of Research & Development of Chinese Medicine, School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.
Microorganisms. 2021 Sep 28;9(10):2052. doi: 10.3390/microorganisms9102052.
The endophytic microbiome is thought to play an important role in promoting plant growth and health. Using culture-independent and culture-dependent protocols, this study characterized the seasonal shifts in the endophytic fungal microbiota of four mulberry ( L.) cultivars having different levels of resistance to mulberry fruit sclerotiniosis. Core endophytes can be obtained by two approaches, and they were divided into two clusters by season. Spring samples harbored higher operational taxonomic units (OTUs) and α-diversity, while autumn samples had more sequences or isolates of the fungal class with the representative orders and . While comparing different mulberry cultivars, we found that the total number of OTUs in susceptible cultivars was higher than that of resistant cultivars, and sp. were observed in all. Notably, the causal agent of fruit sclerotiniosis () was only detected in susceptible cultivars. Collectively, our work elucidated significant variations in the mulberry endophytic microbiome, mainly because of seasonal shifts, and the fact that the host cultivars and mulberry endophytic fungal community appeared to have a certain connection with the resistance level of mulberry fruit to sclerotiniosis. These results provided valuable information on the isolation and culturing of mulberry endophytes that could be applied to improve mulberry fruit production and health.
内生微生物群被认为在促进植物生长和健康方面发挥着重要作用。本研究采用非培养和培养方法，对四个对桑椹菌核病具有不同抗性水平的桑树（L.）品种的内生真菌微生物群的季节性变化进行了表征。核心内生菌可通过两种方法获得，且按季节分为两类。春季样本具有更高的操作分类单元（OTU）和α多样性，而秋季样本具有更多真菌纲的序列或分离物，其代表性目为 和 。在比较不同桑树品种时，我们发现感病品种的OTU总数高于抗病品种，且在所有品种中均观察到 菌。值得注意的是，果实菌核病的病原菌（ ）仅在感病品种中被检测到。总体而言，我们的工作阐明了桑树内生微生物群的显著变化，主要是由于季节性变化，以及宿主品种与桑树内生真菌群落似乎与桑椹对菌核病的抗性水平存在一定联系。这些结果为桑树内生菌的分离和培养提供了有价值的信息，可应用于提高桑椹产量和健康水平。
