Djebaili Rihab, Pellegrini Marika, Ercole Claudia, Farda Beatrice, Kitouni Mahmoud, Del Gallo Maddalena
Department of Life, Health and Environmental Sciences, University of L'Aquila, Coppito, 67100 L'Aquila, Italy.
Laboratory of Microbiological Engineering and Applications, University of Brothers Mentouri Constantine 1, Ain El Bey Road, Constantine 25000, Algeria.
Pathogens. 2021 Oct 12;10(10):1305. doi: 10.3390/pathogens10101305.
Biotic stress caused by pathogenic microorganisms leads to damage in crops. Tomato and carrot are among the most important vegetables cultivated worldwide. These plants are attacked by several pathogens, affecting their growth and productivity. Fourteen plant growth-promoting actinomycetes (PGPA) were screened for their in vitro biocontrol activity against and microbial phytopathogens. Their antifungal activity was evaluated against f. sp. (FORL) and (RHS). Antibacterial activity was evaluated against , , pv. , and subsp. . Strains that showed good in vitro results were further investigated in vitro (cell-free supernatants activity, scanning electron microscope observations of fungal inhibition). The consortium of the most active PGPA was then utilized as biocontrol agents in planta experiments on and . The H12 and H14 strains showed the best in vitro biocontrol activities. The diffusible and volatile compounds and cell-free supernatants of these strains showed both antifungal (in vitro inhibition up to 85%, hyphal desegregation and fungicidal properties) and antibacterial activity (in vitro inhibition >25 mm and bactericidal properties). Their consortium was also able to counteract the infection symptoms of microbial phytopathogens during in planta experiments, improving plant status. The results obtained highlight the efficacy of the selected actinomycetes strains as biocontrol agents of and .
病原微生物引起的生物胁迫会导致作物受损。番茄和胡萝卜是全球种植的最重要蔬菜之一。这些植物受到多种病原体的侵袭，影响其生长和生产力。对14株植物促生放线菌（PGPA）进行了体外生物防治活性筛选，以对抗 和 微生物植物病原体。评估了它们对 f. sp. （FORL）和 （RHS）的抗真菌活性。评估了对 、 、 pv. 和 亚种的抗菌活性。对体外表现良好的菌株进一步进行体外研究（无细胞上清液活性、真菌抑制的扫描电子显微镜观察）。然后将活性最强的PGPA联合体用作 和 的植物体内实验的生物防治剂。 H12和 H14菌株表现出最佳的体外生物防治活性。这些菌株的可扩散和挥发性化合物以及无细胞上清液均表现出抗真菌（体外抑制率高达85%，菌丝解体和杀真菌特性）和抗菌活性（体外抑制>25 mm和杀菌特性）。它们的联合体在植物体内实验中也能够对抗微生物植物病原体的感染症状，改善植物状况。所获得的结果突出了所选放线菌菌株作为 和 的生物防治剂的功效。
