Pellegrini Marika, Ercole Claudia, Gianchino Carmelo, Bernardi Matteo, Pace Loretta, Del Gallo Maddalena
Department of Life, Health and Environmental Sciences, University of L'Aquila, Coppito 1, 67100 L'Aquila, Italy.
Plants (Basel). 2021 Nov 11;10(11):2436. doi: 10.3390/plants10112436.
Industrial hemp ( L.) is a multipurpose plant used in several fields. Several phytopathogens attack hemp crops. is a common fungal pathogen that causes wilt disease in nurseries and in field cultivation and causes high losses. In the present study, a pathogenic strain belonging to f. sp. was isolated from a plant showing wilt. After isolation, identification was conducted based on morphological and molecular characterizations and pathogenicity tests. Selected plant growth-promoting bacteria with interesting biocontrol properties-, , and -were tested against this pathogen. In vitro antagonistic activity was determined by the dual culture method. Effective strains (in vitro inhibition > of 50%) , and were combined in a consortium and screened for in planta antagonistic activity in pre-emergence (before germination) and post-emergence (after germination). The consortium counteracted infection both in pre-emergence and post-emergence. Our preliminary results show that the selected consortium could be further investigated as an effective biocontrol agent for the management of this pathogen.
工业大麻（ Cannabis sativa L.）是一种用于多个领域的多用途植物。几种植物病原体侵袭大麻作物。尖孢镰刀菌（ Fusarium oxysporum ）是一种常见的真菌病原体，可在苗圃和田间种植中引发枯萎病，并造成巨大损失。在本研究中，从一株表现出枯萎症状的植物中分离出了一株属于尖孢镰刀菌大麻专化型（ Fusarium oxysporum f. sp. cannabis ）的致病菌株。分离后，基于形态学、分子特征和致病性测试进行了鉴定。选用具有有趣生防特性的植物促生细菌——解淀粉芽孢杆菌（ Bacillus amyloliquefaciens ）、枯草芽孢杆菌（ Bacillus subtilis ）、巨大芽孢杆菌（ Bacillus megaterium ）和蜡样芽孢杆菌（ Bacillus cereus ）——对该病原体进行测试。通过对峙培养法测定体外拮抗活性。将有效菌株（体外抑制率>50%）解淀粉芽孢杆菌、枯草芽孢杆菌和巨大芽孢杆菌组合成一个联合体，并在出苗前（发芽前）和出苗后（发芽后）筛选其体内拮抗活性。该联合体在出苗前和出苗后均能对抗尖孢镰刀菌的感染。我们的初步结果表明，所选的联合体可作为一种有效的生物防治剂，用于管理这种病原体，值得进一步研究。
