Stewart Adam G, Stewart Alexandra G A
Centre for Clinical Research, Faculty of Medicine, Royal Brisbane and Women's Hospital Campus, The University of Queensland, Brisbane, QLD 4029, Australia.
Department of Infectious Diseases, Royal Brisbane and Women's Hospital, Brisbane, QLD 4029, Australia.
Pathogens. 2021 Oct 13;10(10):1319. doi: 10.3390/pathogens10101319.
species causing human illness are present globally and can cause significant disease. Diagnosis and identification of this intracellular bacteria are challenging with many available diagnostic modalities suffering from several shortcomings. Detection of antibodies directed against spp. via serological methods remains widely used with a broad range of sensitivity and specificity values reported depending on the assay. Molecular methods, including polymerase chain reaction (PCR) testing, enables species-specific identification with a fast turnaround time; however, due to resource requirements, use in some endemic settings is limited. Reports on the use of next-generation sequencing (NGS) and metagenomics to diagnose spp. infection have been increasing. Despite offering several potential advantages in the diagnosis and surveillance of disease, genomic approaches are currently only limited to reference and research laboratories. Continued development of spp. diagnostics is required to improve disease detection and epidemiological surveillance, and to better understand transmission dynamics.
导致人类疾病的物种遍布全球，可引发严重疾病。对这种细胞内细菌进行诊断和鉴定具有挑战性，许多现有的诊断方法都存在一些缺点。通过血清学方法检测针对该菌属的抗体仍然被广泛使用，根据检测方法的不同，报告的敏感性和特异性值范围很广。分子方法，包括聚合酶链反应（PCR）检测，能够快速进行物种特异性鉴定；然而，由于资源需求，在一些流行地区的应用受到限制。关于使用下一代测序（NGS）和宏基因组学来诊断该菌属感染的报告不断增加。尽管基因组方法在疾病诊断和监测方面具有几个潜在优势，但目前仅限于参考实验室和研究实验室。需要持续开发该菌属的诊断方法，以改善疾病检测和流行病学监测，并更好地了解传播动态。
