Department of Nursing, Faculty of Health Sciences, University of Granada, Av. Ilustración, 60, 18016 Granada, Spain.
Department of Food and Health, Instituto de la Grasa-CSIC, Campus of the University Pablo de Olavide, Building 46, 41013 Seville, Spain.
Nutrients. 2021 Sep 29;13(10):3471. doi: 10.3390/nu13103471.
Oleanolic acid (OA), a triterpene that is highly present in olive leaves, has been proposed as a component of functional foods for the prevention of metabolic syndrome, due to its anti-inflammatory activity. We analyzed the effects of OA on inflammatory parameters and signaling proteins in LPS-stimulated THP-1 macrophages. Thus, THP-1 macrophages were incubated with LPS for 48 h after pretreatment with OA at different concentrations. Pretreatment with OA was significantly effective in attenuating IL-6 and TNF-α overproduction induced by LPS in macrophages, and also improved the levels of AMPK-α. We also evaluated the effects of human triglyceride-rich lipoproteins (TRLs) derived from individuals consuming an OA-enriched functional olive oil. For this purpose, TRLs were isolated from healthy adolescents before, 2 and 5 h postprandially after the intake of a meal containing the functional olive oil or common olive oil, and were incubated with THP-1 macrophages. THP-1 macrophages incubated with TRLs isolated at 2 h after the consumption of the OA-enriched olive oil showed significant lower levels of IL-6 compared to the TRLs derived from olive oil. Our results suggest that OA might have potential to be used as a lipid-based formulation in functional olive oils to prevent inflammatory processes underlying metabolic syndrome in adolescents.
齐墩果酸（OA）是一种在橄榄叶中含量很高的三萜烯，因其具有抗炎活性，被提议作为功能性食品的成分，用于预防代谢综合征。我们分析了 OA 对 LPS 刺激的 THP-1 巨噬细胞中炎症参数和信号蛋白的影响。因此，THP-1 巨噬细胞在用不同浓度的 OA 预处理 48 小时后用 LPS 孵育。OA 的预处理可显著有效减轻 LPS 诱导的巨噬细胞中 IL-6 和 TNF-α的过度产生，并改善 AMPK-α的水平。我们还评估了来源于食用富含 OA 的功能性橄榄油的个体的人富含甘油三酯的脂蛋白（TRLs）的影响。为此，在摄入含有功能性橄榄油或普通橄榄油的餐后 2 和 5 小时，从健康青少年中分离 TRLs，并与 THP-1 巨噬细胞孵育。与从橄榄油中分离的 TRL 相比，在用富含 OA 的橄榄油餐后 2 小时孵育的 THP-1 巨噬细胞中，IL-6 的水平显著降低。我们的结果表明，OA 可能有潜力作为功能性橄榄油中的基于脂质的配方，用于预防青少年代谢综合征相关的炎症过程。
