Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea; Plasma Technology Research Center, National Fusion Research Institute, Gunsan-si, Jeollabuk-do, 54004, Republic of Korea.
Plasma Bioscience Research Center, Kwangwoon University, Seoul, 139-701, Republic of Korea.
Arch Biochem Biophys. 2018 Apr 2;643:32-41. doi: 10.1016/j.abb.2018.02.010. Epub 2018 Feb 15.
This study focused on sterilization methods for the reduction of microorganisms on perilla leaves by cylinder type Dielectric Barrier Discharge (DBD) plasma with underwater bubbler treatment. S. aureus and E. coli in a suspension were reduced to less than 3.4 and 0.5 log CFU/ml after the plasma treatment for 3 min, respectively. On the perilla leaves, they were also reduced to 4.8 and 1.6 log CFU/ml after the plasma treatment, respectively. The S. aureus and E. coli bacterial cell wall was damaged by the plasma treatment evident by scanning electron microscopic analysis. The observed infrared bands of the FTIR spectra demonstrated changes in protein, lipid, polysaccharide, polyphosphate group and other carbohydrate functionalities of plasma treated bacteria and untreated bacterial cell membranes. The degradation of the constituent bonds of the bacterial cell membrane by RONS generated from plasma destroys the DNA, RNA, and proteins within the cell, and may eventually cause cell death. In this study, HO (13.68 μM) and NO (138 μM), which are the main factors generated by plasma, proved to have a bactericidal effect by inducing lipid peroxidation of bacterial cell membranes. In conclusion, cylinder type DBD plasma with underwater bubbler can be used as an environmentally friendly food disinfection device in cleaning processes of the food industry.
本研究采用柱状介质阻挡放电（DBD）等离子体与水下鼓泡处理相结合的方法，研究了减少紫苏叶中微生物的灭菌方法。悬液中的金黄色葡萄球菌和大肠杆菌经等离子体处理 3 分钟后，分别减少至<3.4 和 0.5 log CFU/ml。在紫苏叶上，经等离子体处理后，分别减少至 4.8 和 1.6 log CFU/ml。扫描电子显微镜分析表明，等离子体处理破坏了金黄色葡萄球菌和大肠杆菌的细胞壁。傅里叶变换红外光谱（FTIR）的红外带分析表明，等离子体处理后的细菌和未经处理的细菌细胞膜的蛋白质、脂质、多糖、多磷酸盐基团和其他碳水化合物功能发生了变化。等离子体产生的 RONS 降解了细菌细胞膜的组成键，破坏了细胞内的 DNA、RNA 和蛋白质，最终可能导致细胞死亡。在本研究中，HO（13.68 μM）和 NO（138 μM）这两种主要的等离子体生成因子通过诱导细胞膜的脂质过氧化反应被证明具有杀菌作用。总之，柱状 DBD 等离子体与水下鼓泡相结合可以作为一种环保的食品消毒设备，应用于食品工业的清洁过程中。
