Yoon Ji-Seon, Sasaki Ako, Shimada Kenichiroh, Ide Kaori, Iwasaki Toshiroh, Nishifuji Koji
Laboratory of Veterinary Internal Medicine, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan.
Vet Res Commun. 2022 Feb;46(1):121-126. doi: 10.1007/s11259-021-09835-x. Epub 2021 Oct 22.
Ceramide (CER), an important component of the extracellular lamellar lipids in the stratum corneum (SC), plays a critical role in maintaining the cutaneous barrier function. This study aimed to determine whether the quantity of free extractable SC CERs in dogs was affected by the age, sex, or breed. Fifty-eight dogs from the breeds Shiba Inu, beagle, miniature dachshund, shih tzu, and golden retriever, without any history of skin problems, were enrolled in this study. Lipid extracts from the SC were subjected to high-performance thin-layer chromatography to quantify the free extractable CERs. There were weak negative correlations between the age and the amount of free extractable CERs, CER [NP] (non-hydroxy fatty acids linked to phytosphingosines), CER [AS/NH] (α-hydroxy fatty acids linked to sphingosines/non-hydroxy fatty acids linked to 6-hydroxysphingosines), and CER [AP] (α-hydroxy fatty acids and phytosphingosines). There were no significant sex- or breed-related differences in the amounts of free extractable SC CERs in the dogs. These findings imply that aging causes a decline in the amount of free extractable SC CERs in dogs, similar to that observed in humans. The sex or breed of the dogs investigated in this study did not influence the amount of free extractable SC CERs.
神经酰胺（CER）是角质层（SC）细胞外板层脂质的重要组成部分，在维持皮肤屏障功能中起关键作用。本研究旨在确定犬类中可自由提取的SC CERs的量是否受年龄、性别或品种的影响。本研究纳入了58只来自柴犬、比格犬、迷你腊肠犬、西施犬和金毛寻回犬品种且无任何皮肤问题病史的犬只。对SC的脂质提取物进行高效薄层色谱分析，以量化可自由提取的CERs。年龄与可自由提取的CERs、CER [NP]（与植物鞘氨醇相连的非羟基脂肪酸）、CER [AS/NH]（与鞘氨醇相连的α-羟基脂肪酸/与6-羟基鞘氨醇相连的非羟基脂肪酸）和CER [AP]（α-羟基脂肪酸和植物鞘氨醇）的量之间存在弱负相关。犬类中可自由提取的SC CERs的量在性别或品种方面无显著差异。这些发现表明，衰老会导致犬类中可自由提取的SC CERs的量下降，这与在人类中观察到的情况类似。本研究中所调查犬类的性别或品种并未影响可自由提取的SC CERs的量。
