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与健康犬相比，患鱼鳞病的金毛寻回犬角质层中的亚油酸含量降低，且相当一部分以游离和酯化形式被氧化。

The Linoleic Acid Content of the Stratum Corneum of Ichthyotic Golden Retriever Dogs Is Reduced as Compared to Healthy Dogs and a Significant Part Is Oxidized in Both Free and Esterified Forms.

作者信息

Popa Iuliana, Solgadi Audrey, Pin Didier, Watson Adrian L, Haftek Marek, Portoukalian Jacques

机构信息

Analytic and Biological Lipid Systems (Lip(Sys)2), Faculty of Pharmacy, University Paris-Saclay, 92296 Chatenay-Malabry, France.

Technical Platform-Therapeutical Innovation Institute (IPSIT)-UMS 3679 CNRS, Faculty of Pharmacy, University Paris-Saclay, 92290 Chatenay-Malabry, France.

出版信息

Metabolites. 2021 Nov 26;11(12):803. doi: 10.3390/metabo11120803.

DOI:10.3390/metabo11120803
PMID:34940561
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8704365/
Abstract

Golden Retrievers may suffer from -related inherited ichthyosis. Our study shows that in the stratum corneum (SC) of ichthyotic dogs, linoleic acid (LA) is also present in the form of 9-keto-octadecadienoic acid (9-KODE) instead of the acylacid form as in normal dogs. The fatty acids purified from SC strips (LA, acylacids) were characterized by liquid chromatography-tandem mass spectrometry (LC-MS) and atmospheric pressure chemical ionization (APCI). Electrospray ionization (ESI) and MS2(MS/MS Tandem mass spectrum/spectra)/M3 (MS/MS/MS Tandem mass spectrum/spectra) fragmentation indicated the positions of the double bonds in 9-KODE. We showed that ichthyotic dogs have a threefold lower LA content in the form of acylacids. The MS2 fragmentation of acyl acids showed in some peaks the presenceof an ion at the / 279, instead of an ion at / 293 which is characteristic of LA. The detected variant was identified upon MS3 fragmentation as 9-keto-octadecadienoic acid (9-KODE), and the level of this keto-derivative was increased in ichthyotic dogs. We showed by the APCI that such keto forms of LA are produced from hydroperoxy-octadecadienoic acids (HpODE) upon dehydration. In conclusion, the free form of 9-KODE was detected in ichthyotic SC up to fivefold as compared to unaffected dogs, and analyses by HPLC (High performance liquid chromatography) and ESI-MS (Electrospray Ionization-Mass Spectrometry) indicated its production via dehydration of native 9-HpODE.

摘要

金毛寻回犬可能患有与[具体病因未提及]相关的遗传性鱼鳞病。我们的研究表明，在鱼鳞病犬的角质层（SC）中，亚油酸（LA）是以9-酮基-十八碳二烯酸（9-KODE）的形式存在，而不是像正常犬那样以酰基酸的形式存在。从SC条带中纯化的脂肪酸（LA、酰基酸）通过液相色谱-串联质谱（LC-MS）和大气压化学电离（APCI）进行表征。电喷雾电离（ESI）和MS2（MS/MS串联质谱）/M3（MS/MS/MS串联质谱）碎片化表明了9-KODE中双键的位置。我们发现鱼鳞病犬中酰基酸形式的LA含量低三倍。酰基酸的MS2碎片化在一些峰中显示存在m/z 279的离子，而不是LA特有的m/z 293的离子。在MS3碎片化时检测到的变体被鉴定为9-酮基-十八碳二烯酸（9-KODE），并且这种酮衍生物的水平在鱼鳞病犬中有所增加。我们通过APCI表明，LA的这种酮形式是由氢过氧十八碳二烯酸（HpODE）脱水产生的。总之，与未受影响的犬相比，在鱼鳞病的SC中检测到的9-KODE游离形式高达五倍，并且通过高效液相色谱（HPLC）和电喷雾电离质谱（ESI-MS）分析表明其通过天然9-HpODE的脱水产生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/f747653caa25/metabolites-11-00803-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/87081ea9ccf7/metabolites-11-00803-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/37a7720b45da/metabolites-11-00803-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/f8f0365bc47d/metabolites-11-00803-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/62fbfef27f11/metabolites-11-00803-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/b62a168cf3c0/metabolites-11-00803-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/e0aadf11bce5/metabolites-11-00803-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/6137fe330402/metabolites-11-00803-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/f747653caa25/metabolites-11-00803-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/87081ea9ccf7/metabolites-11-00803-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/37a7720b45da/metabolites-11-00803-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/f8f0365bc47d/metabolites-11-00803-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/62fbfef27f11/metabolites-11-00803-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/b62a168cf3c0/metabolites-11-00803-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/e0aadf11bce5/metabolites-11-00803-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/6137fe330402/metabolites-11-00803-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c6/8704365/f747653caa25/metabolites-11-00803-g008.jpg

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