Capitani S, Cocco L, Maraldi N M, Papa S, Manzoli F A
Adv Enzyme Regul. 1986;25:425-38. doi: 10.1016/0065-2571(86)90027-0.
The response of isolated rat liver and murine erythroleukemia nuclei to phospholipid liposomes has been monitored with different techniques, by studying the endogenous RNA synthesis, the release of transcripts in the medium, the pattern of acid-extractable nuclear proteins and the ultra-structural morphology. Total transcription in rat liver and beta-globin mRNA synthesis in MEL nuclei are increased by PS and reduced by PC. These changes of RNA polymerase activity, and the transport of RNAs from nucleus as well as the nuclear protein changes, correlate with structural transitions which occur in both types of nuclei, consisting of euchromatization with loss of RNP particles in the case of PS and opposite effects with PC. The significance of these modifications in relationship to the possible involvement of phospholipids in the control of gene expression is discussed.
通过研究内源性RNA合成、转录本在培养基中的释放、酸可提取核蛋白的模式以及超微结构形态，运用不同技术监测了分离的大鼠肝脏和小鼠红白血病细胞核对磷脂脂质体的反应。PS可增加大鼠肝脏中的总转录以及MEL细胞核中的β-珠蛋白mRNA合成，而PC则使其减少。RNA聚合酶活性的这些变化、RNA从细胞核的转运以及核蛋白的变化，与两种细胞核中发生的结构转变相关，PS处理时发生常染色质化且RNP颗粒丢失，而PC处理则产生相反效果。讨论了这些修饰与磷脂可能参与基因表达调控之间关系的意义。
