Department of Pharmaceutical Science, Faculty of Pharmacy, Airlangga University, Nanizar Zaman Joenoes Building, Jl. Mulyorejo, Surabaya, 60155, East Java, Indonesia; Natural Product Drug Discovery and Development Research Group, Faculty of Pharmacy, Airlangga University, Nanizar Zaman Joenoes Building, Jl. Mulyorejo, Surabaya, 60155, East Java, Indonesia.
Department of Pharmaceutical Science, Faculty of Pharmacy, Airlangga University, Nanizar Zaman Joenoes Building, Jl. Mulyorejo, Surabaya, 60155, East Java, Indonesia.
J Ethnopharmacol. 2022 Feb 10;284:114765. doi: 10.1016/j.jep.2021.114765. Epub 2021 Oct 21.
Graptophyllum pictum (L.) Griff., known as "handeuleum" in West Java and "Daun Ungu" in Indonesia, is traditionally used to cure hemorrhoids.
The purpose of this study is to prove its effectiveness scientifically using anorectal histological parameters in Croton oil-induced hemorrhoid mice.
In vivo tests were performed by observing histomorphologic changes in mice anorectal tissue induced by croton oil. In addition, in vitro assay was performed for evaluating antioxidant activity, astringency property, and hemostasis-associated activity. The antioxidant activity was measured using a DPPH radical scavenging assay. The total flavonoid and phenolic contents were also determined spectrophotometrically.
The in vivo assay showed that the oral-topical combination use of the ethanolic extract of G. pictum leaves demonstrated significant improvement on the croton oil-induced anorectal damage better than the single application by oral or topical application.
These results showed that G. pictum has potent anti hemorrhoid activity, especially for the combinational use of oral and topical administration.
Graptophyllum pictum（L.）Griff.，在西爪哇被称为“handeuleum”，在印度尼西亚被称为“Daun Ungu”，传统上用于治疗痔疮。
本研究旨在通过观察蓖麻油诱导的痔疮小鼠肛直肠组织的组织形态学变化，用肛肠组织的组织形态学参数从科学上证明其疗效。
通过观察蓖麻油诱导的小鼠肛直肠组织的组织形态学变化进行体内试验。此外，还进行了体外试验，以评估抗氧化活性、收敛性和止血相关活性。使用 DPPH 自由基清除测定法测定抗氧化活性。还通过分光光度法测定总黄酮和总酚含量。
体内试验表明，Graptophyllum pictum 叶的乙醇提取物的口服-局部联合应用在改善由蓖麻油引起的肛直肠损伤方面优于口服或局部单一应用。
这些结果表明，Graptophyllum pictum 具有很强的抗痔疮活性，特别是口服和局部联合应用。
