Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Jiangsu Key laboratory of Zoonosis, China.
Anaerobe. 2021 Dec;72:102467. doi: 10.1016/j.anaerobe.2021.102467. Epub 2021 Oct 22.
Clostridium perfringens (C. perfringens) is a common pathogenic microorganism present in nature, which can cause animal and human diseases, such as necrotizing enteritis (NE) in poultry. Little is known about the current prevalence status of C. perfringens from poultry farms of different types and regions in China. From December 2018 to August 2019, we investigated the prevalence, genotype distribution and drug resistance of C. perfringens from Guangdong, Pingyin, Tai'an and Weifang. A total of 622 samples were collected and processed for C. perfringens isolation, among which 239 (38.42%) samples were determined to be positive for C. perfringens. A total of 312 isolates of C. perfringens were recovered (1-5 strains were isolated for each positive sample), and 98.72% of the isolates were identified as type A, while the others were type F. Antimicrobial susceptibility testing revealed that 47.71% of the isolates were resistant to at least five classes of commonly used antibiotics. Multilocus sequence typing (MLST) showed that 74 representative isolates were divided into 63 sequence types (STs), and the Simpson's diversity index (Ds) of the STs for the five farms was 0.9799. 37.84% of the isolates were classified into seven clonal complexes (CC1-CC7), and the isolates from the same farm were more concentrated in the minimum spanning tree. In addition, some cloaca isolates and feed isolates were distributed in the same ST or CC; this result indicates that the C. perfringens in chicken can come from the environment (feed etc.).
产气荚膜梭菌（C. perfringens）是自然界中常见的一种病原微生物，能够引起动物和人类疾病，例如家禽的坏死性肠炎（NE）。目前，关于中国不同类型和地区家禽养殖场中产气荚膜梭菌的流行现状知之甚少。2018 年 12 月至 2019 年 8 月，我们调查了广东、平阴、泰安和潍坊的产气荚膜梭菌的流行情况、基因型分布和耐药性。共采集 622 份样本进行产气荚膜梭菌分离，其中 239 份（38.42%）样本为产气荚膜梭菌阳性。共回收 312 株产气荚膜梭菌（每个阳性样本分离 1-5 株），其中 98.72%鉴定为 A 型，其余为 F 型。药敏试验显示，47.71%的分离株对至少五类常用抗生素耐药。多位点序列分型（MLST）显示，74 株代表性分离株分为 63 个序列型（ST），五个养殖场的 ST 多样性指数（Ds）为 0.9799。37.84%的分离株分为七个克隆复合体（CC1-CC7），同一养殖场的分离株在最小生成树中更为集中。此外，一些泄殖腔分离株和饲料分离株分布在同一 ST 或 CC 中；这一结果表明，鸡体内的产气荚膜梭菌可能来自环境（饲料等）。
