Wu Dan, Luo Runbo, Gong Ga, Zhang Lihong, Huang Jiaqi, Cai Chongzhen, Li Yupeng, Irshad Irfan, Song Rende, Suolang Sizhu
College of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, China.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
Front Vet Sci. 2022 Oct 17;9:1022215. doi: 10.3389/fvets.2022.1022215. eCollection 2022.
is an opportunistic pathogen that cause necrotic enteritis, food poisoning and even death in animals. In this study, we explored the prevalence, antibiotic resistance and genetic diversity of isolated from yak in the Qinghai-Tibet plateau, China. A total of 744 yak fecal samples were collected and assessed for toxin genes, antimicrobial susceptibility and multilocus sequence typing (MLST). Results indicated that 144 out of 744 (19.35%) yak fecal samples were tested to be positive for , 75% ( = 108, 108/144) were type A, 17.36% ( = 25, 25/144) were type C, 2.78% ( = 4, 4/144) were type D, and 4.86% ( = 7, 7/144) were type F. In addition, 2.78% ( = 4, 4/144) of the isolates were positive for toxin gene. Antimicrobial susceptibility testing revealed that 98.61% (142/144) of the isolates showed multiple-antibiotic resistance. According to MLST and phylogenetic tree, 144 yak-derived isolates had an average of 12.95 alleles and could be divided into 89 sequence types (STs) and clustered in 11 clonal complexes (CCs). The most of isolates belong to type A with a considerable genetic diversity, having Simpson index up to 0.9754. MLST and phylogenetic analysis showed that the isolates under the same clade came from multiple regions. Cross-transmission among isolates and interconnectedness were observed in the genetic evolution. According to the study, the most of the isolates exhibited broad-spectrum antibacterial resistance, diverse alleles, and multiple lethal toxin genes of .
是一种机会致病菌，可导致动物坏死性肠炎、食物中毒甚至死亡。在本研究中，我们探索了从中国青藏高原牦牛中分离出的[病菌名称未给出]的流行情况、抗生素抗性和遗传多样性。共收集了744份牦牛粪便样本，并对其毒素基因、抗菌药敏性和多位点序列分型（MLST）进行了评估。结果表明，744份牦牛粪便样本中有144份（19.35%）检测出[病菌名称未给出]呈阳性，75%（n = 108, 108/144）为A型，17.36%（n = 25, 25/144）为C型，2.78%（n = 4, 4/144）为D型，4.86%（n = 7, 7/144）为F型。此外，2.78%（n = 4, 4/144）的分离株[病菌名称未给出]毒素基因呈阳性。抗菌药敏试验显示，98.61%（142/144）的分离株表现出多重抗生素抗性。根据MLST和系统发育树，144株源自牦牛的[病菌名称未给出]分离株平均有12.95个等位基因，可分为89个序列类型（STs），并聚类为11个克隆复合体（CCs）。大多数分离株属于A型，具有相当大的遗传多样性，辛普森指数高达0.9754。MLST和系统发育分析表明，同一进化枝下的分离株来自多个地区。在遗传进化中观察到分离株之间的交叉传播和相互联系。根据该研究，大多数分离株表现出广谱抗菌抗性、多样的等位基因和多种[病菌名称未给出]致死毒素基因。
