Narayanan R, Jastreboff M M, Chiu C F, Bertino J R
Biochem Biophys Res Commun. 1986 Dec 30;141(3):1018-24. doi: 10.1016/s0006-291x(86)80146-2.
Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.
