Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza60714-903, CE, Brazil.
Laboratory of Biotechnology and Molecular Biology (LBBM), State University of Ceará, Fortaleza60714-903, CE, Brazil.
Zygote. 2022 Jun;30(3):419-422. doi: 10.1017/S0967199421000794. Epub 2021 Oct 25.
The aim of this study was to evaluate the effect of 1 µmol/l zearalenone (ZEN) and 1 µmol/l matairesinol (MAT), alone or in combination, on the morphology of in vitro-cultured ovarian preantral follicles. Ovaries from four adult sheep were collected at a local slaughterhouse and fragmented, and the ovarian pieces were submitted to in vitro culture for 3 days in the presence or absence of the test compounds. The morphology of primordial and primary follicles was impaired by ZEN. The plant lignan MAT alone did not maintain the morphology of the ovarian follicles; its combination with ZEN counteracted the negative effects observed when follicles were cultured in the presence of the mycotoxin alone. However, MAT was not able to promote the in vitro development of the ovarian follicles.
本研究旨在评估 1µmol/L 的玉米赤霉烯酮（ZEN）和 1µmol/L 的马尾松素（MAT）单独或联合作用对体外培养的卵巢原始卵泡形态的影响。本研究从当地屠宰场采集了四只成年绵羊的卵巢并进行了碎片化处理，然后将卵巢组织片段在存在或不存在测试化合物的情况下进行体外培养 3 天。ZEN 会损害原始卵泡和初级卵泡的形态。植物木质素 MAT 单独使用不能维持卵巢卵泡的形态；当卵泡在单独存在真菌毒素的情况下培养时，MAT 与 ZEN 的联合使用可以抵消观察到的负面影响。然而，MAT 不能促进卵巢卵泡的体外发育。
