Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, 60714-903, CE, Brazil.
Laboratory of Biotechnology and Molecular Biology (LBBM), State University of Ceará, Fortaleza, 60714-903, CE, Brazil.
Theriogenology. 2021 Oct 15;174:124-130. doi: 10.1016/j.theriogenology.2021.08.025. Epub 2021 Aug 20.
The aim of this study was to evaluate the effect of 1 μmol/L zearalenone (ZEN) and 1 μmol/L enterolactone (ENL), alone or in combination, on the survival and morphology of in vitro cultured ovarian preantral follicles. Ovaries from 10 sheep were collected at a local abattoir and fragmented, and the ovarian pieces were submitted to in vitro culture for 3 days in the presence or absence of the test compounds. The morphology of primordial and primary follicles was impaired by ZEN, whereas that of cultured secondary follicles was improved by ENL. However, the combination of ENL with ZEN impaired the quality of primary and secondary follicles. Both ZEN and ENL induced apoptosis, but only ZEN was responsible for oocyte autophagy. None of these xenoestrogens affected endoplasmic reticulum stress as observed by the unaltered expression of ERP29. Differently from ZEN, ENL increased the expression of the efflux transporter ABCG2. In conclusion, although ENL can counteract the negative effects of ZEN on primordial and primary follicles, this positive effect is not similar to that observed in ovarian tissue cultures in the presence of ENL alone.
本研究旨在评估 1 μmol/L 玉米赤霉烯酮（ZEN）和 1 μmol/L 肠内酯（ENL）单独或联合对体外培养卵巢小腔前卵泡存活和形态的影响。从当地屠宰场采集 10 只绵羊的卵巢并进行碎片化处理，将卵巢碎片在存在或不存在测试化合物的情况下进行 3 天的体外培养。ZEN 损害原始和初级卵泡的形态，而 ENL 则改善次级卵泡的培养形态。然而，ENL 与 ZEN 的联合使用损害了初级和次级卵泡的质量。ZEN 和 ENL 均诱导细胞凋亡，但只有 ZEN 导致卵母细胞自噬。这些外源性雌激素均未引起内质网应激，ERP29 的表达未改变。与 ZEN 不同，ENL 增加了外排转运蛋白 ABCG2 的表达。总之，尽管 ENL 可以抵消 ZEN 对原始和初级卵泡的负面影响，但这种积极作用与单独存在 ENL 时在卵巢组织培养中观察到的作用不同。
