PG and Research Centre in Biotechnology, MGR College, Hosur, Tamilnadu, India.
PG and Centre for Research in Botany, Thiagarajar College, Madurai, Tamilnadu, India.
J Infect Public Health. 2022 Feb;15(2):297-306. doi: 10.1016/j.jiph.2021.10.012. Epub 2021 Oct 14.
The emergence of resistance to commonly used antibiotics by human infections occurred mostly due to their overuse, that prompted individuals to pursue novel and innovative treatments. The phytochemical characteristics, antibacterial activity, and cytotoxicity of MCF7 cells were evaluated in two Pleurotus spp. mycelial extracts in this work.
Pleurotus djamor and Pleurotus florida mycelial extracts from pure cultures were tested for antibacterial activity by a well-diffusion assay and antimicrobial activity against mold fungi was evaluated for biomass inhibition. Mycelial extracts were obtained from dichloromethane extracts and their biophysical characteristics are analyzed by UV-vis spectrum and FTIR analysis. By spraying detection reagents onto TLC plates, the chemicals in dichloromethane extraction of chosen mushroom fungus mycelia were identified. Using the MTT test, the cytotoxic effect of dichloromethane extracts of selected mushroom fungi was evaluated on MCF7 Cell lines.
Mycelial extracts of P. djamor and P. florida exhibited significant antimicrobial effect on the bacterial and fungal pathogens tested. Dichloromethane mycelial extracts were obtained using soxhlet extraction which response positive for various phytochemical analysis. Detection of metabolites in thin layer chromatography using spray reagents documented one of few first accounts on flavonoids, anthroquinone and terpenoid compounds in P. djamor and P. florida. P. djamor and P. florida had dose-dependent antiproliferative activity against MCF7 cells, with an inhibitory impact of 55.72% and 64.47% percent at 125 μg/mL, respectively.
The study has reported the identification with the potent biological activity of some of the key bioactive components present in DCM extracts from the mycelia of P. djamor and P. florida.
人类感染对常用抗生素产生耐药性主要是由于过度使用，这促使人们寻求新的创新治疗方法。本工作评估了两种侧耳属真菌菌丝体提取物的植物化学成分、抗菌活性和对 MCF7 细胞的细胞毒性。
通过平板扩散法测试了纯培养的糙皮侧耳和佛罗里达侧耳菌丝体提取物的抗菌活性，并评估了对霉菌真菌的生物量抑制的抗菌活性。从二氯甲烷提取物中获得菌丝体提取物，并通过紫外可见光谱和傅里叶变换红外（FTIR）分析分析其生物物理特性。通过将检测试剂喷洒到 TLC 板上，鉴定了选定蘑菇真菌菌丝体二氯甲烷提取物中的化学物质。使用 MTT 试验评估了选定蘑菇真菌二氯甲烷提取物对 MCF7 细胞系的细胞毒性作用。
糙皮侧耳和佛罗里达侧耳的菌丝体提取物对测试的细菌和真菌病原体表现出显著的抗菌作用。使用索氏提取法获得二氯甲烷菌丝体提取物，该提取物对各种植物化学分析呈阳性反应。使用喷雾试剂在薄层色谱中检测代谢物记录了糙皮侧耳和佛罗里达侧耳中黄酮类、蒽醌和萜类化合物的少数首次报道之一。糙皮侧耳和佛罗里达侧耳对 MCF7 细胞具有剂量依赖性的抗增殖活性，在 125μg/mL 时分别具有 55.72%和 64.47%的抑制作用。
本研究报告了从糙皮侧耳和佛罗里达侧耳的菌丝体中二氯甲烷提取物中存在的一些关键生物活性成分的鉴定及其潜在的生物活性。
