Ajuga Martha Uzoaru, Otokunefor Kome, Agbagwa Obakpororo Ejiro
Department of Microbiology, Abia State Polytechnic, P.M.B 7166, Aba, Nigeria.
Department of Microbiology, University of Port Harcourt, P.M.B.5323, Port Harcourt, Nigeria.
Bull Natl Res Cent. 2021;45(1):173. doi: 10.1186/s42269-021-00628-5. Epub 2021 Oct 19.
The increase in multidrug resistance (MDR) among pathogenic bacteria responsible for infectious diseases has led to lack of effectiveness of some antibiotics. The ability of to harbor resistant genes has made the treatment of infections a major challenge. This study was carried out to assess antibiotic resistance and extended-spectrum beta-lactamase (ESBL) production of E. coli from various sources in Aba metropolis, Nigeria.
From a total of 350 samples collected from clinical and non-clinical sources, 137 were presumptively identified as by standard phenotypic methods and 83 were confirmed as by the detection of specific 16S rRNA gene fragments. The majority of these isolates (52, 62.7%) were from non-clinical sources. The clinical isolates, however, exhibited a higher level of resistance against 62.5% of tested antibiotics. Both group of isolates exhibited similar levels (58.1% vs 53.9%) of MDR, though. A low rate of ESBL production was observed (1.2%) following phenotypic detection of ESBL-producing abilities using the double-disc synergy test. An assessment of the presence of three beta-lactamase gene genotypes (bla, bla and bla) revealed that none of the three predominant ESBL genotypes was identified in this study.
This study reports high levels of antibiotic resistance in both clinical and non-clinical isolates. Though higher rates of resistance were observed among the non-clinical isolates, both group of organisms had similar levels of MDR. Strikingly, however, was the low level of ESBL producers detected in this study and the absence of the three main genotypes associated with ESBL production in this study.
导致传染病的致病细菌中多重耐药性（MDR）的增加已导致一些抗生素失效。携带耐药基因的能力使感染治疗成为一项重大挑战。本研究旨在评估尼日利亚阿巴市不同来源大肠杆菌的抗生素耐药性及超广谱β-内酰胺酶（ESBL）的产生情况。
从临床和非临床来源共收集350份样本，通过标准表型方法初步鉴定出137株为大肠杆菌，通过检测特定的16S rRNA基因片段确认其中83株为大肠杆菌。这些分离株大多数（52株，62.7%）来自非临床来源。然而，临床分离株对62.5%的测试抗生素表现出更高水平的耐药性。不过，两组分离株的多重耐药水平相似（分别为58.1%和53.9%）。使用双纸片协同试验对产ESBL能力进行表型检测后，观察到ESBL产生率较低（1.2%）。对三种β-内酰胺酶基因基因型（bla TEM、bla SHV和bla CTX-M）存在情况的评估显示，本研究未鉴定出三种主要的ESBL基因型中的任何一种。
本研究报告了临床和非临床大肠杆菌分离株中高水平的抗生素耐药性。虽然在非临床分离株中观察到更高的耐药率，但两组生物体的多重耐药水平相似。然而，本研究中检测到的ESBL产生菌水平较低以及本研究中未出现与ESBL产生相关的三种主要基因型，这一点令人惊讶！
