Phenotypic and Molecular Characterization of -Lactamases among Enterobacterial Uropathogens in Southeastern Nigeria.

Little is known about the molecular basis of antibiotic resistance among uropathogens in Southeast Nigeria. The aim of the study was to characterize enterobacterial uropathogens with respect to drug resistance. One hundred (100) enterobacterial uropathogens were studied. Their antibiotic susceptibility patterns were evaluated using disk diffusion, screened, and confirmed phenotypically for the presence of -lactamases: ESBL, AmpC, carbapenemase, and MBLs. Screen positives were further tested for various -lactamase genes by PCR. Our isolates showed variable resistance to most drugs tested. Out of the 58 ESBL screen positive 35 were confirmed positive with PCR. The predominant ESBL gene was bla while bla was the most prevalent among MBL genes. Forty-six percentage of the screen positive isolates coharbored bla genes. Nine of the 10 ESBL screen positive were phenotypically and PCR positive. Three isolates of were positive for MBL genes. All the 10  were positive for ESBL genes. The study showed high prevalence of drug-resistant genes among the enterobacterial uropathogens. Majority of the uropathogens harbored >1 antibiotic-resistant gene, and the most predominant gene was ESBL (bla) followed by the MBL (SPM) gene.