Cooke H M, Penketh R J, Delhanty J D
Clin Genet. 1986 Dec;30(6):485-93. doi: 10.1111/j.1399-0004.1986.tb01916.x.
Culture of chorionic villus cells provides a method of obtaining chromosomes of excellent quality for first trimester prenatal diagnosis. Concern exists that maternal cells present in the biopsy may contaminate the culture and lead to misdiagnosis. This study has confirmed that karyotypes obtained from female villus cultures were non-maternal by establishing the presence of paternal markers using Q- and C-banding. Male cultures were harvested serially to investigate the possibility of maternal cell overgrowth. Of 82 successful cultures investigated, 37 were male and 45 female and of the males 4 contained a mixture of male and female cells. Thorough dissection of the material is essential if maternal cell contamination is to be mininimised. The use of heteromorphic chromosome markers to establish that fetal cells have grown provides valuable reassurance.
绒毛膜绒毛细胞培养为孕早期产前诊断提供了一种获取高质量染色体的方法。人们担心活检中存在的母体细胞可能会污染培养物并导致误诊。本研究通过使用Q带和C带确定父系标记的存在，证实了从女性绒毛培养物中获得的核型并非来自母体。对男性培养物进行连续收获，以研究母体细胞过度生长的可能性。在研究的82例成功培养物中，37例为男性，45例为女性，其中4例男性培养物含有男性和女性细胞的混合物。如果要将母体细胞污染降至最低，对材料进行彻底解剖至关重要。使用异形染色体标记来确定胎儿细胞已经生长，可提供有价值的保证。
