从……中进行三相盐析萃取富含二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的油脂
Three-liquid-phase salting-out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)-rich oils from .
作者信息
Zeb Liaqat, Teng Xin-Nan, Shafiq Muhammad, Wang Shu-Chang, Xiu Zhi-Long, Su Zhi-Guo
机构信息
School of Bioengineering Dalian University of Technology Dalian P. R. China.
State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. China.
出版信息
Eng Life Sci. 2021 Jul 26;21(10):666-682. doi: 10.1002/elsc.202000098. eCollection 2021 Oct.
The TLPSOES parameters were optimized by response surface methodology using Box-Behnken design, which were 16.5% w/w of ammonium citrate, 17.5% w/w of ethanol, and 46% w/w of n-hexane at 70 min of stirring time. Under optimized conditions the extraction efficiency attained was 90.91 ± 0.97% of EPA, 90.02 ± 1.04% of DHA, and 91.85 ± 1.11% of KO in the top n-hexane phase. The highest extraction efficiency of proteins and flavonoids, i.e. 88.34 ± 1.35% and 79.67 ± 1.13%, was recorded in the solid interface and ethanol phase, respectively. The KO extracted by TLPSOES system consisted of lowest fluoride level compared to the conventional method and whole wet krill biomass. The TLPSOES is a potential candidate for nutraceutical industry of KO extraction from wet krill biomass.
采用Box-Behnken设计的响应面法对三相液-液-固萃取系统（TLPSOES）参数进行了优化，在搅拌时间70分钟时，柠檬酸铵为16.5%（w/w）、乙醇为17.5%（w/w）、正己烷为46%（w/w）。在优化条件下，正己烷上层相中二十碳五烯酸（EPA）的萃取效率达到90.91±0.97%，二十二碳六烯酸（DHA）的萃取效率达到90.02±1.04%，角鲨烯（KO）的萃取效率达到91.85±1.11%。蛋白质和类黄酮的最高萃取效率分别为88.34±1.35%和79.67±1.13%，分别出现在固体界面和乙醇相中。与传统方法和整个湿磷虾生物质相比，通过三相液-液-固萃取系统提取的角鲨烯的氟含量最低。三相液-液-固萃取系统是从湿磷虾生物质中提取角鲨烯的营养保健品行业的潜在候选方法。
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