González-Benítez Natalia, Martín-Rodríguez Irene, Cuesta Isabel, Arrayás Manuel, White James Francis, Molina María Carmen
Department of Biology, Geology, Physics, and Inorganic Chemistry, Universidad Rey Juan Carlos, Madrid, Spain.
Unidad de Bioinformática, Instituto de Salud Carlos III, Madrid, Spain.
Front Microbiol. 2021 Oct 6;12:664271. doi: 10.3389/fmicb.2021.664271. eCollection 2021.
Seed microbiota is becoming an emergent area of research. Host plant microbial diversity is increasingly well described, yet relatively little is known about the stressors driving plant endomicrobiota at the metaorganism level. The present work examines the role of horizontal and vertical transmission of bacterial microbiota in response to abiotic stress generated by arsenic. Horizontal transmission is achieved by bioaugmentation with the endophyte , while vertical transmission comes maternal inheritance from seeds. To achieve this goal, all experiments were conducted with two species. is tolerant to arsenic (As), whereas , being phylogenetically close to , was not previously described as As tolerant. The core bacterial endophytes are composed of genera , , , , and and family across different environmental conditions. All these operational taxonomic units (OTUs) coexisted from seeds to the development of the seedling, independently of As stress, or bioaugmentation treatment and species. colonized efficiently both species, driving the endomicrobiota structure of with a stronger effect than As stress. Despite the fact that most of the OTUs identified inside seeds and seedlings belonged to rare microbiota, they represent a large bacterial reservoir offering important physiological and ecological traits to the host. traits co-regulated with , and the associated microbiota improved the host response to As stress. NGS-Illumina tools provided further knowledge about the ecological and functional roles of plant endophytes.
种子微生物群正在成为一个新兴的研究领域。宿主植物的微生物多样性已得到越来越充分的描述，但在元生物体水平上，关于驱动植物内生微生物群的应激源却知之甚少。本研究探讨了细菌微生物群的水平和垂直传播在应对砷产生的非生物胁迫中的作用。水平传播通过用内生菌进行生物强化来实现，而垂直传播则来自种子的母体遗传。为实现这一目标，所有实验均使用两个物种进行。[物种名称1]对砷（As）具有耐受性，而[物种名称2]虽然在系统发育上与[物种名称1]相近，但之前并未被描述为耐砷。在不同环境条件下，核心细菌内生菌由[属名1]、[属名2]、[属名3]、[属名4]、[属名5]属以及[科名]科组成。所有这些可操作分类单元（OTU）从种子到幼苗发育阶段都共存，与砷胁迫、生物强化处理以及[物种名称]无关。[内生菌名称]有效地定殖于两个物种，对[物种名称1]内生微生物群结构的驱动作用比砷胁迫更强。尽管在[物种名称1]种子和幼苗中鉴定出的大多数OTU属于稀有微生物群，但它们代表了一个庞大的细菌库，为宿主提供了重要的生理和生态特性。[特性名称]与[内生菌名称]共同调节，并且相关的微生物群改善了宿主对砷胁迫的反应。NGS-Illumina工具提供了关于植物内生菌生态和功能作用的更多知识。
