Ferreira Anderson, Quecine Maria Carolina, Lacava Paulo Teixeira, Oda Shinitiro, Azevedo João Lúcio, Araújo Welington Luiz
Departmento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, Brazil.
FEMS Microbiol Lett. 2008 Oct;287(1):8-14. doi: 10.1111/j.1574-6968.2008.01258.x.
The diversity and beneficial characteristics of endophytic microorganisms have been studied in several host plants. However, information regarding naturally occurring seed-associated endophytes and vertical transmission among different life-history stages of hosts is limited. Endophytic bacteria were isolated from seeds and seedlings of 10 Eucalyptus species and two hybrids. The results showed that endophytic bacteria, such as Bacillus, Enterococcus, Paenibacillus and Methylobacterium, are vertically transferred from seeds to seedlings. In addition, the endophytic bacterium Pantoea agglomerans was tagged with the gfp gene, inoculated into seeds and further reisolated from seedlings. These results suggested a novel approach to change the profile of the plants, where the bacterium is a delivery vehicle for desired traits. This is the first report of an endophytic bacterial community residing in Eucalyptus seeds and the transmission of these bacteria from seeds to seedlings. The bacterial species reported in this work have been described as providing benefits to host plants. Therefore, we suggest that endophytic bacteria can be transmitted vertically from seeds to seedlings, assuring the support of the bacterial community in the host plant.
内生微生物的多样性和有益特性已在多种宿主植物中得到研究。然而，关于自然存在的与种子相关的内生菌以及宿主不同生活史阶段之间的垂直传播的信息有限。从10种桉树和两个杂交种的种子及幼苗中分离出内生细菌。结果表明，芽孢杆菌、肠球菌、类芽孢杆菌和甲基杆菌等内生细菌可从种子垂直传播至幼苗。此外，将成团泛菌用绿色荧光蛋白基因标记，接种到种子中，然后从幼苗中再次分离出来。这些结果提示了一种改变植物特性的新方法，即细菌可作为所需性状的传递载体。这是关于桉树种子中存在内生细菌群落以及这些细菌从种子向幼苗传播的首次报道。本研究中报道的细菌物种已被描述为对宿主植物有益。因此，我们认为内生细菌可从种子垂直传播至幼苗，确保宿主植物中细菌群落的支持。
