Yang Zhendong, Jin Jianping, Chang Tao
Department of E.N.T, The Ninth People's Hospital of Suzhou, Suzhou 215200, Jiangsu, China.
J Oncol. 2021 Oct 13;2021:2408384. doi: 10.1155/2021/2408384. eCollection 2021.
Laryngeal cancer accounts for 20% of all head and neck malignancies. Laryngeal squamous cell carcinoma (LSCC) is the most common type of laryngeal cancer and is characterized by squamous differentiation, a high mortality rate, and poor prognosis. Accumulating studies have indicated that circular RNAs (circRNAs) are critical regulators in many cancers. CircPTK2 exerts an important regulatory role in several cancers. In this study, we aimed to elucidate the function of circPTK2 (hsa_circ_0003221) in LSCC. Through a series of investigations, we discovered that circPTK2 was significantly upregulated in LSCC tissues cells. Functionally, cell counting kit-8 (CCK-8) and flow cytometry analyses revealed that knockdown of circPTK2 suppressed LSCC cell viability and the cell cycle while promoting cell apoptosis. Notably, silencing circPTK2 inhibited tumor growth . Mechanistically, circPTK2 functioned as a molecular sponge of miR-1278 to upregulate YAP1 expression in LSCC cells. Moreover, YAP1 knockdown inhibited malignant phenotypes of LSCC cells. The rescue experiments showed that YAP1 overexpression reversed the effects of circPTK2 on LSCC cells. Therefore, we concluded that circPTK2 facilitates LSCC progression through the miR-1278/YAP1 axis.
喉癌占所有头颈部恶性肿瘤的20%。喉鳞状细胞癌（LSCC）是喉癌最常见的类型，其特征为鳞状分化、高死亡率和预后不良。越来越多的研究表明，环状RNA（circRNAs）是许多癌症中的关键调节因子。CircPTK2在几种癌症中发挥重要的调节作用。在本研究中，我们旨在阐明circPTK2（hsa_circ_0003221）在LSCC中的功能。通过一系列研究，我们发现circPTK2在LSCC组织细胞中显著上调。在功能上，细胞计数试剂盒-8（CCK-8）和流式细胞术分析显示，敲低circPTK2可抑制LSCC细胞活力和细胞周期，同时促进细胞凋亡。值得注意的是，沉默circPTK2可抑制肿瘤生长。机制上，circPTK2作为miR-1278的分子海绵，上调LSCC细胞中YAP1的表达。此外，敲低YAP1可抑制LSCC细胞的恶性表型。挽救实验表明，YAP1过表达可逆转circPTK2对LSCC细胞的影响。因此，我们得出结论，circPTK2通过miR-1278/YAP1轴促进LSCC进展。
