The Department of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
The Department of Computer, Xi'an University of Post and Telecommunications, Xi'an, 710121, China.
Mol Cell Probes. 2020 Aug;52:101574. doi: 10.1016/j.mcp.2020.101574. Epub 2020 Apr 11.
MicroRNAs (miRNAs), a type of post-transcriptional regulators, exert a crucial role in the malignant progression of laryngeal squamous cell carcinoma (LSCC). MicroRNA-330-3p (miR-330-3p), a recently identified tumor-associated miRNA, is implicated in multiple cancers. Yet, the relevance of miR-330-3p in LSCC remains unexplored. The findings of our study demonstrated a lower expression of miR-330-3p in LSCC. Functional assays revealed that upregulation of miR-330-3p marked restricted the proliferation, colony formation and invasion of LSCC cells. Transformer-2 protein homolog beta (Tra2β) was identified as a target gene of miR-330-3p. An inverse correlation between miR-330-3p and Tra2β mRNA expression was evidenced in LSCC specimens. The upregulation of miR-330-3p significantly repressed Tra2β expression and the phosphorylation of the Akt protein. In addition, Tra2β overexpression markedly abrogated the tumor suppressive role of miR-330-3p in LSCC cells. Overall, our results uncovered that miR-330-3p exerted a tumor-inhibition function in LSCC through targeting Tra2β to inhibit Akt activation.
微小 RNA（miRNAs）是一种转录后调控因子，在喉鳞状细胞癌（LSCC）的恶性进展中发挥着关键作用。MicroRNA-330-3p（miR-330-3p）是最近发现的一种与肿瘤相关的 miRNA，它与多种癌症有关。然而，miR-330-3p 在 LSCC 中的相关性尚未得到探索。我们的研究结果表明，miR-330-3p 在 LSCC 中的表达较低。功能分析表明，miR-330-3p 的上调显著限制了 LSCC 细胞的增殖、集落形成和侵袭。Transformer-2 蛋白同源物β（Tra2β）被鉴定为 miR-330-3p 的靶基因。LSCC 标本中证明了 miR-330-3p 与 Tra2β mRNA 表达之间存在负相关。miR-330-3p 的上调显著抑制了 Tra2β 的表达和 Akt 蛋白的磷酸化。此外，Tra2β 的过表达显著削弱了 miR-330-3p 在 LSCC 细胞中的肿瘤抑制作用。总之，我们的研究结果表明，miR-330-3p 通过靶向 Tra2β 抑制 Akt 激活，在 LSCC 中发挥肿瘤抑制作用。
