环状 RNA 0067934 通过海绵吸附 miR-1324 与不良预后相关，并促进喉鳞状细胞癌进展。
Circ_0067934 correlates with poor prognosis and promotes laryngeal squamous cell cancer progression by sponging miR-1324.
机构信息
Department of Otorhinolaryngology, Air Force Hospital of the Northern Theater of the People's Liberation Army, Shenyang, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Apr;24(8):4320-4327. doi: 10.26355/eurrev_202004_21013.
OBJECTIVE
As a kind of non-coding RNA, circular RNA (circRNA) plays a regulatory role in many tumors. Our intention was to investigate the clinical significance, biological function, and molecular regulation mechanisms of circ_0067934 in laryngeal squamous cell cancer (LSCC) progression.
PATIENTS AND METHODS
The expression levels of circ_0067934 in LSCC tumor samples and cells were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The associations of circ_0067934 expression with clinicopathological features and overall survival in LSCC patients were statistically analyzed. The biological function of circ_0067934 in LSCC cells was analyzed by CCK-8, colony formation, EdU, and transwell assays. Dual-Luciferase reporter assay was conducted to verify that circ_0067934 may sponge miR-1324 to regulate proliferation and migration of LSCC.
RESULTS
Circ_0067934 expression was remarkably upregulated in LSCC tissues and cells. High level of circ_0067934 was significantly related to tumor size, lymph node status, and distant metastasis of LSCC. Furthermore, highly expressed circ_0067934 could result in notably worse survival. In vitro experiments showed that down-regulation of circ_0067934 inhibited proliferation and migration of LSCC cells. Mechanism analysis revealed that circ_0067934 regulated LSCC development by sponging miR-1324.
CONCLUSIONS
Circ_0067934 may function as an oncogene in LSCC, which provided a feasible prognostic biomarker and therapeutic target for LSCC.
目的
作为一种非编码 RNA，环状 RNA（circRNA）在许多肿瘤中发挥着调控作用。我们旨在研究 circ_0067934 在喉鳞状细胞癌（LSCC）进展中的临床意义、生物学功能和分子调控机制。
患者和方法
通过实时定量聚合酶链反应（qRT-PCR）测定 LSCC 肿瘤标本和细胞中 circ_0067934 的表达水平。统计分析 circ_0067934 表达与 LSCC 患者临床病理特征和总生存的相关性。通过 CCK-8、集落形成、EdU 和 Transwell 测定分析 circ_0067934 在 LSCC 细胞中的生物学功能。双荧光素酶报告实验验证 circ_0067934 可能通过海绵吸附 miR-1324 来调节 LSCC 的增殖和迁移。
结果
circ_0067934 在 LSCC 组织和细胞中表达明显上调。高水平的 circ_0067934 与 LSCC 的肿瘤大小、淋巴结状态和远处转移显著相关。此外，高表达的 circ_0067934 可导致明显较差的生存。体外实验表明，circ_0067934 的下调抑制了 LSCC 细胞的增殖和迁移。机制分析表明，circ_0067934 通过海绵吸附 miR-1324 调节 LSCC 的发生发展。
结论
circ_0067934 可能在 LSCC 中作为癌基因发挥作用，为 LSCC 提供了一种可行的预后生物标志物和治疗靶点。
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