Parmiani G, Taramelli D, Anichini A, Andreola S, Cascinelli N, Fossati G
J Immunogenet. 1986 Apr-Jun;13(2-3):235-40. doi: 10.1111/j.1744-313x.1986.tb01106.x.
The expression of class I and class II HLA antigens has been studied on primary and metastatic human melanomas, and on cell clones derived from the latter. A panel of monoclonal antibodies and flow cytofluorometric analysis were used to evaluate the presence of HLA-A, B, C and -DR, DQ antigens on freshly isolated tumour cells. HLA class I antigens were present on 91% and 93% of primary and metastatic tumours, respectively. Sixty per cent of primary and 50% of metastatic melanomas expressed HLA-DR antigens, whereas 38% and 21% of cases were positive for HLA-DQ. A marked heterogeneity was evident among primary and metastatic lesions for expression of class I and II antigens. Similar findings were obtained by analysing the phenotype of melanoma clones which indicates that a marked antigenic heterogeneity for class I and II HLA antigens occurs even among clones isolated from short-term cultures of metastatic melanomas.
已对原发性和转移性人类黑色素瘤以及源自后者的细胞克隆进行了I类和II类HLA抗原表达的研究。使用一组单克隆抗体和流式细胞荧光分析来评估新鲜分离的肿瘤细胞上HLA-A、B、C和-DR、DQ抗原的存在情况。I类HLA抗原分别存在于91%的原发性肿瘤和93%的转移性肿瘤中。60%的原发性黑色素瘤和50%的转移性黑色素瘤表达HLA-DR抗原，而38%和21%的病例HLA-DQ呈阳性。I类和II类抗原在原发性和转移性病变中的表达存在明显异质性。通过分析黑色素瘤克隆的表型也获得了类似的结果，这表明即使在从转移性黑色素瘤短期培养物中分离出的克隆中，I类和II类HLA抗原也存在明显的抗原异质性。
