全球阿尔茨海默病协会血浆淀粉样蛋白β检测方法循环研究
The global Alzheimer's Association round robin study on plasma amyloid β methods.
作者信息
Pannee Josef, Shaw Leslie M, Korecka Magdalena, Waligorska Teresa, Teunissen Charlotte E, Stoops Erik, Vanderstichele Hugo M J, Mauroo Kimberley, Verberk Inge M W, Keshavan Ashvini, Pesini Pedro, Sarasa Leticia, Pascual-Lucas Maria, Fandos Noelia, Allué José-Antonio, Portelius Erik, Andreasson Ulf, Yoda Ritsuko, Nakamura Akinori, Kaneko Naoki, Yang Shieh-Yueh, Liu Huei-Chun, Palme Stefan, Bittner Tobias, Mawuenyega Kwasi G, Ovod Vitaliy, Bollinger James, Bateman Randall J, Li Yan, Dage Jeffrey L, Stomrud Erik, Hansson Oskar, Schott Jonathan M, Blennow Kaj, Zetterberg Henrik
机构信息
Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology The Sahlgrenska Academy, University of Gothenburg Mölndal Sweden.
Clinical Neurochemistry Lab Sahlgrenska University Hospital Mölndal Sweden.
出版信息
Alzheimers Dement (Amst). 2021 Oct 14;13(1):e12242. doi: 10.1002/dad2.12242. eCollection 2021.
INTRODUCTION
Blood-based assays to measure brain amyloid beta (Aβ) deposition are an attractive alternative to the cerebrospinal fluid (CSF)-based assays currently used in clinical settings. In this study, we examined different blood-based assays to measure Aβ and how they compare among centers and assays.
METHODS
Aliquots from 81 plasma samples were distributed to 10 participating centers. Seven immunological assays and four mass-spectrometric methods were used to measure plasma Aβ concentrations.
RESULTS
Correlations were weak for Aβ42 while Aβ40 correlations were stronger. The ratio Aβ42/Aβ40 did not improve the correlations and showed weak correlations.
DISCUSSION
The poor correlations for Aβ42 in plasma might have several potential explanations, such as the high levels of plasma proteins (compared to CSF), sensitivity to pre-analytical sample handling and specificity, and cross-reactivity of different antibodies. Different methods might also measure different pools of plasma Aβ42. We, however, hypothesize that greater correlations might be seen in future studies because many of the methods have been refined during completion of this study.
引言
用于测量脑淀粉样β蛋白（Aβ）沉积的血液检测方法是目前临床中使用的基于脑脊液（CSF）检测方法的一种有吸引力的替代方法。在本研究中，我们检测了不同的用于测量Aβ的血液检测方法，以及它们在不同中心和检测方法之间的比较情况。
方法
从81份血浆样本中取出的等分试样被分发给10个参与中心。使用七种免疫检测方法和四种质谱方法来测量血浆Aβ浓度。
结果
Aβ42的相关性较弱，而Aβ40的相关性较强。Aβ42/Aβ40比值并未改善相关性，且显示出较弱的相关性。
讨论
血浆中Aβ42相关性较差可能有多种潜在解释，例如血浆蛋白水平较高（与脑脊液相比）、对分析前样本处理的敏感性和特异性以及不同抗体的交叉反应性。不同方法也可能测量不同的血浆Aβ42池。然而，我们推测在未来的研究中可能会看到更高的相关性，因为许多方法在本研究完成期间已经得到改进。
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