College of Veterinary Medicine, South China Agricultural University, Guangzhou, PR China.
The GBA National Institute for Nanotechnology Innovation, Guangzhou, PR China.
J Food Biochem. 2021 Dec;45(12):e13974. doi: 10.1111/jfbc.13974. Epub 2021 Oct 25.
This study aimed to explore the protective effect of sulfated Codonopsis polysaccharides (SCP) on acute oxidative stress. SCP was modified by chlorosulfonic acid-pyridine method from Codonopsis polysaccharides (CP), which had 34.48% of sulfate content determined by ultrasonic-acidic barium chromate spectrophotometry. The analysis of Fourier transform-infrared spectroscopy (FT-IR) appeared an absorption peak of SCP at 811.91 cm , which related to C-O-SO . In vitro test, the antioxidant activities of CP and SCP was induced by H O in RAW264.7 cells, results indicated that SCP and CP could significantly enhance the activity of superoxide dismutase (SOD), glutathione peroxidase (GDH-Px) and catalase (CAT), and nitric oxide (NO) and decrease the level of malondialdehyde (MDA), reactive oxygen species (ROS), and inducible nitric oxide synthase (iNOS) secreted by RAW264.7 cells compared with modeling group (p < .05). The flow cytometry results also revealed that SCP and CP could markedly inhibit the apoptosis of macrophage induced by acute oxidative stress. In vivo test, 50% ethanol was used to induce mice acute oxidative stress, results indicated that the blood biochemical parameters in mice were restored to normal levels following administration of SCP and CP, and alanine aminotransferase (ALT), aspartate transaminase (AST), total protein (TP), albumin (ALB), glucose (GLU), and creatinine (UREA) had significant differences compared with modeling group (p < .05). Quantitative real-time PCR analysis revealed that SCP and CP could promote the expression of Keap1 and Nrf2. In summary, both SCP and CP had protective effects against acute oxidative stress. PRACTICAL APPLICATIONS: Oxidative stress is a kind of stress injury, which can cause a variety of diseases and accelerate physical aging. Codonopsis has many active components, among which Codonopsis polysaccharide has antioxidant effect. Recent studies have found that Codonopsis polysaccharides could be modified by sulfate molecules to obtain higher antioxidant activity. The modified Codonopsis polysaccharides could significantly promote the production of antioxidant enzymes (SOD, CAT, GDH-Px) and reduce the content of oxidative stress marks (ROS, MDA). Moreover, its antioxidant mechanism may be related to the Keap1 /Nrf2 signaling pathway. Therefore, SCP was an effective antioxidant, and could be used as a potential health food with antioxidant and anti-aging effects.
本研究旨在探讨硫酸化党参多糖（SCP）对急性氧化应激的保护作用。SCP 是通过氯磺酸-吡啶法从党参多糖（CP）中修饰得到的，其硫酸基含量通过超声-酸性重铬酸钡分光光度法测定为 34.48%。傅里叶变换-红外光谱（FT-IR）分析显示，SCP 在 811.91cm-1 处出现一个吸收峰，与 C-O-SO 有关。体外试验中，H2O2 诱导 RAW264.7 细胞产生的 CP 和 SCP 的抗氧化活性表明，SCP 和 CP 可显著增强超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GDH-Px）和过氧化氢酶（CAT）的活性，降低 RAW264.7 细胞中丙二醛（MDA）、活性氧（ROS）和诱导型一氧化氮合酶（iNOS）的水平，与模型组相比（p<0.05）。流式细胞术结果还表明，SCP 和 CP 可显著抑制急性氧化应激诱导的巨噬细胞凋亡。体内试验中，50%乙醇诱导小鼠急性氧化应激，结果表明 SCP 和 CP 可使小鼠血液生化参数恢复正常水平，与模型组相比，丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总蛋白（TP）、白蛋白（ALB）、葡萄糖（GLU）和肌酐（UREA）均有显著差异（p<0.05）。实时定量 PCR 分析显示，SCP 和 CP 可促进 Keap1 和 Nrf2 的表达。综上所述，SCP 和 CP 对急性氧化应激均有保护作用。实际应用：氧化应激是一种应激损伤，可导致多种疾病并加速身体衰老。党参含有多种活性成分，其中党参多糖具有抗氧化作用。最近的研究发现，党参多糖可以通过硫酸分子修饰得到，从而获得更高的抗氧化活性。修饰后的党参多糖能显著促进抗氧化酶（SOD、CAT、GDH-Px）的产生，降低氧化应激标志物（ROS、MDA）的含量。此外，其抗氧化机制可能与 Keap1/Nrf2 信号通路有关。因此，SCP 是一种有效的抗氧化剂，可作为一种具有抗氧化和抗衰老作用的潜在保健品。
